Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human placenta tissue using MBS7000171 at dilution of 1:100)
Rabbit anti-Human PIH1D1 Polyclonal Antibody | anti-PIH1D1 antibody
PIH1D1 Antibody
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human placenta tissue using MBS7000171 at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human placenta tissue using MBS7000171 at dilution of 1:100)
Immunofluorescence (IF)
(Immunofluorescence staining of Hela cells cells with MBS7000171 at 1:200,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Immunofluorescence (IF)
(Immunofluorescence staining of Hela cells cells with MBS7000171 at 1:200,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Immunoprecipitation (IP)
(Immunoprecipitating PIH1D1 in Hela whole cell lysate Lane 1: Rabbit control IgG (1ug)instead of MBS7000171 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: MBS7000171 (6ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (10ug))
Immunoprecipitation (IP)
(Immunoprecipitating PIH1D1 in Hela whole cell lysate Lane 1: Rabbit control IgG (1ug)instead of MBS7000171 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: MBS7000171 (6ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (10ug))
NCBI and Uniprot Product Information
Uniprot Description
PIH1D1: a protein of unknown function.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 19q13.33
Cellular Component: cytoplasm; nucleolus; nucleus
Molecular Function: ATPase binding; histone binding; phosphoprotein binding; protein binding; protein kinase binding
Biological Process: box C/D snoRNP assembly; chromatin remodeling; epithelial cell differentiation; positive regulation of protein complex assembly; regulation of histone H3-K4 methylation; snoRNA localization; transcription, DNA-dependent
Research Articles on PIH1D1
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Product Notes
The PIH1D1 pih1d1 (Catalog #AAA7000171) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PIH1D1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PIH1D1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Recommended dilution: WB:1:500-2000, IHC:1:20-1:200. Researchers should empirically determine the suitability of the PIH1D1 pih1d1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIH1D1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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