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Western Blot (WB) (PIGS Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PIGS antibody detected the PIGS protein (arrow).)

Rabbit anti-Human PIGS Polyclonal Antibody | anti-PIGS antibody

PIGS, CT (PIGS, GPI transamidase component PIG-S, Phosphatidylinositol-glycan biosynthesis class S protein) (AP)

Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PIGS; Polyclonal Antibody; CT (PIGS; GPI transamidase component PIG-S; Phosphatidylinositol-glycan biosynthesis class S protein) (AP); Phosphatidylinositol-glycan biosynthesis class S protein; anti-PIGS antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-PIGS antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
PIGS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 526-555 amino acids from the C-terminal region of human PIGS.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PIGS Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PIGS antibody detected the PIGS protein (arrow).)

Western Blot (WB) (PIGS Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PIGS antibody detected the PIGS protein (arrow).)
Related Product Information for anti-PIGS antibody
This gene encodes a protein that is involved in GPI-anchor biosynthesis. The glycosylphosphatidylinositol (GPI) anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This gene encodes an essential component of the multisubunit enzyme, GPI transamidase. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. [provided by RefSeq].
Product Categories/Family for anti-PIGS antibody
References
Vainauskas, S., et al. J. Biol. Chem. 280(16):16402-16409(2005). Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003). Hong, Y., et al. Mol. Biol. Cell 14(5):1780-1789(2003). Ohishi, K., et al. J. Biol. Chem. 278(16):13959-13967(2003). Vainauskas, S., et al. J. Biol. Chem. 277(34):30535-30542(2002).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
61,005 Da
NCBI Official Full Name
GPI transamidase component PIG-S
NCBI Official Synonym Full Names
phosphatidylinositol glycan anchor biosynthesis class S
NCBI Official Symbol
PIGS
NCBI Protein Information
GPI transamidase component PIG-S
UniProt Protein Name
GPI transamidase component PIG-S
UniProt Gene Name
PIGS

NCBI Description

This gene encodes a protein that is involved in GPI-anchor biosynthesis. The glycosylphosphatidylinositol (GPI) anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This gene encodes an essential component of the multisubunit enzyme, GPI transamidase. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

PIGS: Component of the GPI transamidase complex. Essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates. Belongs to the PIGS family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: endoplasmic reticulum membrane; GPI-anchor transamidase complex; membrane

Molecular Function: GPI-anchor transamidase activity; protein binding

Biological Process: attachment of GPI anchor to protein

Similar Products

Product Notes

The PIGS pigs (Catalog #AAA6333893) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PIGS, CT (PIGS, GPI transamidase component PIG-S, Phosphatidylinositol-glycan biosynthesis class S protein) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PIGS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PIGS pigs for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIGS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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