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Western Blot (WB) (Western blot analysis of PIG-Y in human skeletal muscle tissue lysate with PIG-Y antibody at 5 μg/mL.)

Rabbit anti-Human, Mouse PIG-Y Polyclonal Antibody | anti-PIGY antibody

PIG-Y Antibody

Gene Names
PYURF; PREY
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
PIG-Y Antibody is affinity chromatography purified via peptide column.
Synonyms
PIG-Y; Polyclonal Antibody; PIG-Y Antibody; Phosphatidylinositol N-acetylglucosaminyltransferase subunit Y; Phosphatidylinositol-glycan biosynthesis class Y protein; phosphatidylinositol glycan anchor biosynthesis; class Y; anti-PIGY antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
This antibody only detects isoform 1 of PIG-Y.
Purity/Purification
PIG-Y Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
114
Applicable Applications for anti-PIGY antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
PIG-Y antibody can be used for detection of PIG-Y by Western blot at 2 mug/mL. Despite its predicted molecular weight, PIG-Y often migrates at 28-30 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
PIG-Y antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human PIG-Y.
Buffer
PIG-Y Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
PIG-Y antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of PIG-Y in human skeletal muscle tissue lysate with PIG-Y antibody at 5 μg/mL.)

Western Blot (WB) (Western blot analysis of PIG-Y in human skeletal muscle tissue lysate with PIG-Y antibody at 5 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of PIGY in human spleen tissue with PIGY antibody at 2.5 μg/mL.)

Immunohistochemistry (IHC) (Immunohistochemistry of PIGY in human spleen tissue with PIGY antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of PIGY in human spleen tissue with PIGY1 antibody at 20 μg/mL.)

Immunofluorescence (IF) (Immunofluorescence of PIGY in human spleen tissue with PIGY1 antibody at 20 μg/mL.)
Related Product Information for anti-PIGY antibody
PIG-Y Antibody: Glycosylphosphatidylinositol (GPI) lipid anchoring is an important post-translational modification of proteins that takes place in the endoplasmic reticulum. The synthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), a complex of proteins including PIG-A, PIG-H, PIG-C, GPI1, and DPM2. PIG-Y, the mammalian homolog to yeast Eri1p, is also thought to be involved in the biosynthesis of GPI. The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
12,655 Da
NCBI Official Full Name
protein preY, mitochondrial
NCBI Official Synonym Full Names
PIGY upstream reading frame
NCBI Official Symbol
PYURF
NCBI Official Synonym Symbols
PREY
NCBI Protein Information
protein preY, mitochondrial; PIGY upstream reading frame protein
UniProt Protein Name
Protein preY, mitochondrial
UniProt Gene Name
PYURF
UniProt Synonym Gene Names
PREY
UniProt Entry Name
PREY_HUMAN

NCBI Description

The product of this gene, which is well-conserved, is encoded by the same bicistronic transcript that encodes phosphatidylinositol glycan anchor biosynthesis, class Y, but the two proteins are unrelated. This gene represents the protein encoded by the upstream open reading frame, while the protein encoded by the downstream open reading frame is represented by GeneID:84992. [provided by RefSeq, Aug 2012]

Uniprot Description

PIGY: Component of the GPI-GlcNAc transferase (GPI-GnT) complex in the endoplasmic reticulum, a complex that catalyzes transfer of GlcNAc from UDP-GlcNAc to an acceptor phosphatidylinositol, the first step in the production of GPI-anchors for cell surface proteins. May act by regulating the catalytic subunit PIGA. Belongs to the PREY family.

Protein type: Membrane protein, multi-pass; Endoplasmic reticulum; Membrane protein, integral; Mitochondrial

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex; endoplasmic reticulum membrane; integral to membrane; plasma membrane

Biological Process: GPI anchor biosynthetic process

Similar Products

Product Notes

The PIGY pyurf (Catalog #AAA151115) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PIG-Y Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PIG-Y can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). PIG-Y antibody can be used for detection of PIG-Y by Western blot at 2 mug/mL. Despite its predicted molecular weight, PIG-Y often migrates at 28-30 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the PIGY pyurf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIG-Y, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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