Western Blot (WB)
(Western Blot (WB) analysis of PIF1 polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:PC12 whole cell lysate)
Rabbit PIF1 Polyclonal Antibody | anti-PIF1 antibody
PIF1 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
PIF1; Polyclonal Antibody; PIF1 Polyclonal Antibody; ATP-dependent DNA helicase PIF1; DNA repair and recombination helicase PIF-1; PIF1/RRM3 DNA helicase-like protein; C15orf20; anti-PIF1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
PIF1 polyclonal antibody detects endogenous levels of PIF1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
641
Applicable Applications for anti-PIF1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
A synthetic peptide corresponding to residues in Human PIF1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of PIF1 polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:PC12 whole cell lysate)
Western Blot (WB)
(Western Blot (WB) analysis of PIF1 polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:PC12 whole cell lysate)
Related Product Information for anti-PIF1 antibody
PIF1 is a single-stranded (ss) DNA-dependent ATPase as well as a DNA helicase that unwinds DNA in a 5' to 3' direction with respect to the DNA strand on which it binds first. This protein is critical to DNA replication and telomere length maintenance in Saccharomyces cerevisiae. The PIF1 gene is highly conserved from yeast to humans. Mutations in the gene that encodes for PIF1 cause all telomeres to lengthen, suggesting that the PIF1 functions as a catalytic inhibitor of both new telomere formation and telomere elongation. Human PIF1 preferentially binds telomeric DNA where it inhibits telomerase activity. PIF1 specifically counteracts the RecQ homolog Sgs1 helicase activity, and degradation of PIF1 is mediated by the ubiquitin-26S proteasome pathway.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
~ 70kDa
NCBI Official Full Name
ATP-dependent DNA helicase PIF1
UniProt Protein Name
ATP-dependent DNA helicase PIF1
Protein Family
UniProt Gene Name
PIF1
UniProt Entry Name
PIF1_HUMAN
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Product Notes
The PIF1 pif1 (Catalog #AAA3006390) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PIF1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PIF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the PIF1 pif1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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