Rabbit anti-Human PIASy Polyclonal Antibody | anti-PIASy antibody

PIASy, NT (PIAS4, PIASG, E3 SUMO-protein ligase PIAS4, PIASy, Protein inhibitor of activated STAT protein 4, Protein inhibitor of activated STAT protein gamma) (APC)

Cat. Num. AAA6333784

• Gene Names: PIAS4; PIASY; Piasg; ZMIZ6
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PIASy; Polyclonal Antibody; NT (PIAS4; PIASG; E3 SUMO-protein ligase PIAS4; Protein inhibitor of activated STAT protein 4; Protein inhibitor of activated STAT protein gamma) (APC); PIAS4; Protein inhibitor of activated STAT protein gamma; anti-PIASy antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-PIASy antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PIASy antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56~86 amino acids from the N-terminal region of human PIASy1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(The anti-PIASy polyclonal antibody is used in Western blot to detect PIASy in HL-60 cell lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-PIASy antibody

PIASy1 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role in transcriptional coregulation of various cellular pathways, including the STAT pathway, the p53 pathway, the wnt pathway and the steroid hormone signaling pathway. PIASy1 is involved in gene silencing, and promotes PARK7 sumoylation.

Product Categories/Family for anti-PIASy antibody

Antibodies; Transcription Factors; STAT

References

Imoto, S., et al., J. Biol. Chem. 278(36):34253-34258 (2003). Chun, T.H., et al., Circ. Res. 92(11):1201-1208 (2003). Subramanian, L., et al., J. Biol. Chem. 278(11):9134-9141 (2003). Liu, B., et al., Proc. Natl. Acad. Sci. U.S.A. 98(6):3203-3207 (2001). Liu, B., et al., Proc. Natl. Acad. Sci. U.S.A. 95(18):10626-10631 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 24850133
• NCBI GeneID: 51588
• NCBI Accession #: NP_056981.2
• NCBI GenBank Nucleotide #: NM_015897.2
• UniProt Accession #: Q8N2W9; O75926; Q96G19; Q9UN16
• Molecular Weight: 56,504 Da
• NCBI Official Full Name: E3 SUMO-protein ligase PIAS4
• NCBI Official Synonym Full Names: protein inhibitor of activated STAT, 4
• NCBI Official Symbol: PIAS4
• NCBI Official Synonym Symbols: PIASY; Piasg; ZMIZ6
• NCBI Protein Information: E3 SUMO-protein ligase PIAS4; PIAS-gamma; zinc finger, MIZ-type containing 6; protein inhibitor of activated STAT protein 4; protein inhibitor of activated STAT protein PIASy; protein inhibitor of activated STAT protein gamma
• UniProt Protein Name: E3 SUMO-protein ligase PIAS4
• UniProt Gene Name: PIAS4
• UniProt Synonym Gene Names: PIASG; PIAS-gamma
• UniProt Entry Name: PIAS4_HUMAN

Uniprot Description

PIAS4: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Promotes PARK7 sumoylation. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Interacts with AR, AXIN1, GATA2, LEF1, TP53 and STAT1 (IFNG-induced). Binds to AT-rich DNA sequences, known as matrix or scaffold attachment regions (MARs/SARs). Interacts with TICAM1. Interacts with KLF8; the interaction results in SUMO ligation and repression of KLF8 transcriptional activity and of its cell cycle progression into G(1) phase. Highly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes. Belongs to the PIAS family.

Protein type: EC 6.3.2.-; SUMO conjugating system; Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; PML body; nuclear matrix; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; ubiquitin protein ligase binding; SUMO ligase activity; ligase activity

Biological Process: positive regulation of protein sumoylation; Wnt receptor signaling pathway; protein sumoylation; transcription, DNA-dependent; inhibition of NF-kappaB transcription factor; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent