Rabbit PIAS, gamma Polyclonal Antibody | anti-Pias4 antibody

PIAS, gamma (Protein Inhibitors of Activated STAT)

Cat. Num. AAA614796

• Gene Names: Pias4; PIASY; Piasg; Pias-gamma
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: PIAS; gamma; Polyclonal Antibody; gamma (Protein Inhibitors of Activated STAT); Anti -PIAS; anti-Pias4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human. Species Crossreactivity: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.

• Applicable Applications for anti-Pias4 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1-2ug/ml.
• Immunogen: Synthetic peptides corresponding to amino acids 44-60 and 187-198 of human PIASg. These sequences are 95% and 100% homologous in mouse and rat.
• Positive Control: Jurkat cell lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of PIASg in Jurkat cell lysate using IMG-290 at 2 ug/ml.)

Related Product Information for anti-Pias4 antibody

The interaction of a cytokine with its specific cell surface receptor triggers the activation of intracellular signaling pathways that ultimately program the cellular response. The signal transducers and activators of transcription (STAT) proteins play important roles in the activation of these pathways. Recently, a family of proteins has been identified that inhibits activated STATS and are named as Protein Inhibitors of Activated STAT (PIAS). PIAS proteins comprise a family of five mammalian proteins, which have been identified as potentially important down regulators of the STAT-signaling pathway. The cDNA for mouse and human PIASg encodes a protein of 507 amino acids that is highly homologous to the human protein and is expressed in the mouse as early as d 7.5 of gestation. In situ hybridizations of staged mouse embryos localized the transcript for the PIASgamma gene to the limbs, neuroepithelium, and the inner root sheath of the hair follicle, suggesting a role in the development of these structures. This protein is also present in hair follicle of human scalp hair and in monkey neuronal cells. Thus PIASg exhibits a highly selective pattern of expression, suggesting that it modulates the response of cells to developmental cues.

Product Categories/Family for anti-Pias4 antibody

Antibodies; Abs to Transcription Factors; STAT

NCBI and Uniprot Product Information

• NCBI GI #: 10178613
• NCBI GeneID: 59004
• UniProt Accession #: Q9JM05; Q8R165
• Molecular Weight: 55,570 Da
• NCBI Official Full Name: protein inhibitor of activated STAT protein gamma
• NCBI Official Synonym Full Names: protein inhibitor of activated STAT 4
• NCBI Official Symbol: Pias4
• NCBI Official Synonym Symbols: PIASY; Piasg; Pias-gamma
• NCBI Protein Information: E3 SUMO-protein ligase PIAS4; protein inhibitor of activated STAT PIASy; protein inhibitor of activated STAT protein 4; protein inhibitor of activated STAT protein gamma
• UniProt Protein Name: E3 SUMO-protein ligase PIAS4
• Protein Family: E3 SUMO-protein ligase
• UniProt Gene Name: Pias4
• UniProt Synonym Gene Names: Piasg; PIAS-gamma
• UniProt Entry Name: PIAS4_MOUSE

Uniprot Description

PIAS4: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Promotes PARK7 sumoylation. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Interacts with AR, AXIN1, GATA2, LEF1, TP53 and STAT1 (IFNG-induced). Binds to AT-rich DNA sequences, known as matrix or scaffold attachment regions (MARs/SARs). Interacts with TICAM1. Interacts with KLF8; the interaction results in SUMO ligation and repression of KLF8 transcriptional activity and of its cell cycle progression into G(1) phase. Highly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes. Belongs to the PIAS family.

Protein type: EC 6.3.2.-; Nuclear receptor co-regulator; Transcription, coactivator/corepressor; SUMO conjugating system

Cellular Component: nucleoplasm; nuclear matrix; cytoplasm; intracellular; nucleus

Molecular Function: protein C-terminus binding; protein binding; DNA binding; zinc ion binding; coenzyme F420-2 alpha-glutamyl ligase activity; metal ion binding; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; ubiquitin protein ligase binding; ribosomal S6-glutamic acid ligase activity; coenzyme F420-0 gamma-glutamyl ligase activity; transcription corepressor activity; SUMO ligase activity; ligase activity

Biological Process: positive regulation of protein sumoylation; Wnt receptor signaling pathway; protein sumoylation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; viral reproduction; inhibition of NF-kappaB transcription factor; multicellular organismal development; negative regulation of transcription from RNA polymerase II promoter; JAK-STAT cascade; negative regulation of transcription, DNA-dependent