Rabbit anti-Human PI3KC3 Polyclonal Antibody | anti-PIK3C3 antibody

PI3KC3 Antibody (S425)

Cat. Num. AAA9202650

• Gene Names: PIK3C3; VPS34; Vps34; hVps34
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: PI3KC3; Polyclonal Antibody; PI3KC3 Antibody (S425); Phosphatidylinositol 3-kinase catalytic subunit type 3; PI3-kinase type 3; PI3K type 3; PtdIns-3-kinase type 3; Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide-3-kinase class 3; hVps34; PIK3C3; VPS34; anti-PIK3C3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This PI3KC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 405-430 amino acids from human PI3KC3.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.25 (varies by lot)
• Sequence Positions: 405-430
• Sequence Length: 887

• Applicable Applications for anti-PIK3C3 antibody: ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of Phospho-PI3KC3-pS425 in MDA-MB231 cell line lysates (35ug/lane). PI3KC3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PI3KC3 Antibody (S425), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-PIK3C3 antibody

PI3KC3 is a catalytic subunit of the PI3K complex involved in the transport of lysosomal enzyme precursors to lysosomes. This enzyme acts catalytically to convert 1-phosphatidyl-1D-myo-inositol to 1-phosphatidyl-1D-myo-inositol 3-phosphate.

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The regulation of the Beclin 1-PI3KC3 complex lipid kinase activity is a critical element in the autophagy signaling pathway.

Product Categories/Family for anti-PIK3C3 antibody

Immunology; Metabolism; Signal Transduction; GPCR Antibodies

References

Vergne, I., et al., J. Exp. Med. 198(4):653-659 (2003).
Volinia, S., et al., EMBO J. 14(14):3339-3348 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 34761064
• NCBI GeneID: 5289
• NCBI Accession #: NP_002638.2
• NCBI GenBank Nucleotide #: NM_002647.3
• UniProt Accession #: Q8NEB9; Q15134
• Molecular Weight: 101549
• NCBI Official Full Name: phosphatidylinositol 3-kinase catalytic subunit type 3 isoform 1
• NCBI Official Synonym Full Names: phosphatidylinositol 3-kinase, catalytic subunit type 3
• NCBI Official Symbol: PIK3C3
• NCBI Official Synonym Symbols: VPS34; Vps34; hVps34
• NCBI Protein Information: phosphatidylinositol 3-kinase catalytic subunit type 3
• UniProt Protein Name: Phosphatidylinositol 3-kinase catalytic subunit type 3
• UniProt Gene Name: PIK3C3
• UniProt Synonym Gene Names: VPS34; PI3-kinase type 3; PI3K type 3; PtdIns-3-kinase type 3
• UniProt Entry Name: PK3C3_HUMAN

Uniprot Description

PIK3C3: Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. Required for transport from early to late endosomes. Heterodimer. This subunit, part of a complex composed of regulatory and catalytic subunits, associates with regulatory subunit PIK3R4. Forms a complex with BECN1, PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. In this complex, presence of UVRAG and ATG14 are mutually exclusive. Part of a complex composed of PIK3R4 and PIK3CB. Interacts with RAB7A in the presence of PIK3R4. Ubiquitously expressed, with a highest expression in skeletal muscle. Belongs to the PI3/PI4-kinase family.

Protein type: Kinase, lipid; EC 2.7.1.137; Motility/polarity/chemotaxis; Autophagy; Carbohydrate Metabolism - inositol phosphate

Chromosomal Location of Human Ortholog: 18q12.3

Cellular Component: membrane; late endosome; axoneme; phagocytic vesicle; midbody; cytosol; phosphoinositide 3-kinase complex

Molecular Function: protein binding; 1-phosphatidylinositol-3-kinase activity; ATP binding; protein kinase activity

Biological Process: phosphoinositide-mediated signaling; endosome organization and biogenesis; cytokinesis; protein amino acid phosphorylation; regulation of protein secretion; response to leucine; early endosome to late endosome transport; phosphoinositide phosphorylation; phospholipid metabolic process; phosphatidylinositol biosynthetic process; toll-like receptor signaling pathway; insulin receptor signaling pathway; innate immune response; protein processing; toll-like receptor 9 signaling pathway; autophagic vacuole formation