Rabbit anti-Human PI3KC2G Polyclonal Antibody | anti-PI3KC2G antibody

PI3KC2G, NT (Phosphatidylinositol-4-phosphate 3-kinase C2 Domain-containing Subunit gamma, Phosphoinositide 3-kinase-C2-gamma, PI3K-C2gamma, PI3K-C2-gamma, PIK3C2G, PtdIns-3-kinase C2 Subunit gamma, MGC163149) (Azide free) (HRP)

Cat. Num. AAA6493491

• Gene Names: PIK3C2G; PI3K-C2GAMMA; PI3K-C2-gamma
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PI3KC2G; Polyclonal Antibody; NT (Phosphatidylinositol-4-phosphate 3-kinase C2 Domain-containing Subunit gamma; Phosphoinositide 3-kinase-C2-gamma; PI3K-C2gamma; PI3K-C2-gamma; PIK3C2G; PtdIns-3-kinase C2 Subunit gamma; MGC163149) (Azide free) (HRP); Phosphoinositide-3-kinase Class 2 gamma Polypeptide; EC=2.7.1.154; anti-PI3KC2G antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PI3KC2G.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PI3KC2G antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human PI3KC2G (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PI3KC2G antibody (N-term) in CEM cell line lysates (35ug/lane). PI3KC2G (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-PI3KC2G antibody

Antibodies; Protein Kinases

References

1. Rozycka, M., et al., Genomics 54(3):569-574 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 571026668
• NCBI GeneID: 5288
• NCBI Accession #: NP_001275701.1
• NCBI GenBank Nucleotide #: NM_001288772.1
• UniProt Accession #: O75747; A1L3U0
• Molecular Weight: 165,715 Da
• NCBI Official Full Name: phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit gamma isoform 1
• NCBI Official Synonym Full Names: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 gamma
• NCBI Official Symbol: PIK3C2G
• NCBI Official Synonym Symbols: PI3K-C2GAMMA; PI3K-C2-gamma
• NCBI Protein Information: phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit gamma
• UniProt Protein Name: Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit gamma
• UniProt Gene Name: PIK3C2G
• UniProt Synonym Gene Names: PI3K-C2-gamma; PtdIns-3-kinase C2 subunit gamma

NCBI Description

The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. This gene may play a role in several diseases, including type II diabetes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

PIK3C2G: Generates phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) that act as second messengers. May play a role in SDF1A-stimulated chemotaxis. Belongs to the PI3/PI4-kinase family.

Protein type: Carbohydrate Metabolism - inositol phosphate; EC 2.7.1.154; Kinase, lipid; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12p12.3

Cellular Component: cytoplasm; cytosol; phosphoinositide 3-kinase complex; plasma membrane

Molecular Function: 1-phosphatidylinositol-3-kinase activity; ATP binding; phosphatidylinositol binding; phosphatidylinositol phosphate kinase activity; phosphatidylinositol-4-phosphate 3-kinase activity

Biological Process: cell migration; chemotaxis; phosphatidylinositol biosynthetic process; phosphatidylinositol phosphorylation; phosphoinositide 3-kinase cascade