Rabbit PI3K gamma/Pik3cg Polyclonal Antibody | anti-Pik3cg antibody

Anti-PI3K gamma/Pik3cg Antibody, Rabbit Polyclonal

Cat. Num. AAA8100854

• Gene Names: PIK3CG; PI3K; PIK3; PI3CG; PI3Kgamma
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: PI3K gamma/Pik3cg; Polyclonal Antibody; Anti-PI3K gamma/Pik3cg Antibody; Rabbit Polyclonal; Pik3cg/PIK3CG Antibody; Rabbit PAb; Antigen Affinity Purified; anti-Pik3cg antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PI3K gamma/Pik3cg
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-Pik3cg antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human Pik3cg/PIK3CG
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Pik3cg/PIK3CG, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human PIK3CG in human liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human PIK3CG in human kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human PIK3CG in mouse liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human PIK3CG in mouse skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-Pik3cg antibody

Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by this gene is a class I catalytic subunit of PI3K. Like other class I catalytic subunits (p11-alpha p11-beta, and p11-delta), the encoded protein binds a p85 regulatory subunit to form PI3K. This gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. Several transcript variants encoding the same protein have been found for this gene.

Product Categories/Family for anti-Pik3cg antibody

Common G-Chain signaling pathway

References

Kachele M, Hennige AM, Machann J, et al. Variation in the Phosphoinositide 3-Kinase Gamma Gene Affects Plasma HDL-Cholesterol without Modification of Metabolic or Inflammatory Markers. Zhang Y, ed. PLoS ONE. 2015;10(12):e0144494.

NCBI and Uniprot Product Information

• NCBI GI #: 21237725
• NCBI GeneID: 5294
• NCBI Accession #: NP_002640.2
• NCBI GenBank Nucleotide #: NM_002649.2
• UniProt Accession #: P48736; Q8IV23; Q9BZC8; A4D0Q6
• Molecular Weight: 126,454 Da
• NCBI Official Full Name: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform
• NCBI Official Synonym Full Names: phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit gamma
• NCBI Official Symbol: PIK3CG
• NCBI Official Synonym Symbols: PI3K; PIK3; PI3CG; PI3Kgamma
• NCBI Protein Information: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform; p110gamma; p120-PI3K; PI3K-gamma; p110-gamma; PI3-kinase subunit gamma; ptdIns-3-kinase subunit gamma; 1-phosphatidylinositol 3-kinase; ptdIns-3-kinase subunit p110-gamma; ser
• UniProt Protein Name: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform
• Protein Family: Phosphatidylinositol 4,5-bisphosphate 3-kinase
• UniProt Gene Name: PIK3CG
• UniProt Synonym Gene Names: PI3-kinase subunit gamma; PI3K-gamma; PI3Kgamma; PtdIns-3-kinase subunit gamma; PtdIns-3-kinase subunit p110-gamma; p110gamma
• UniProt Entry Name: PK3CG_HUMAN

NCBI Description

This gene encodes a protein that belongs to the pi3/pi4-kinase family of proteins. The gene product is an enzyme that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. It is an important modulator of extracellular signals, including those elicited by E-cadherin-mediated cell-cell adhesion, which plays an important role in maintenance of the structural and functional integrity of epithelia. In addition to its role in promoting assembly of adherens junctions, the protein is thought to play a pivotal role in the regulation of cytotoxicity in NK cells. The gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. [provided by RefSeq, Jul 2008]

Uniprot Description

PIK3CG: catalytic subunit of the PI3/PI4-kinase family of phospholipid-kinases. Phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. Phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 recruits proteins with PH domains to the membrane, including AKT1 and PDPK1, and plays a critical pleiotropic role in regulating membrane signaling. Activated by both the alpha and the beta:gamma G proteins following stimulation of G protein-coupled receptors (GPCRs), linking GPCR activation to PIP3 production. Activation by GPCRs is assisted by the regulatory subunits (PIK3R5 or PIK3R6), leading to the translocation from the cytosol to the plasma membrane. Inhibited by AS-604850 and AS-605240. Activates signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in immune, inflammatory and allergic responses. Modulates leukocyte chemotaxis to inflammatory sites and in response to chemoattractant agents. May control leukocyte polarization and migration by regulating the spatial accumulation of PIP3 and by regulating the organization of F-actin formation and integrin-based adhesion at the leading edge. Controls motility of dendritic cells. Together with PIK3CD, it regulates many cellular functions: it is involved in natural killer (NK) cell development and migration towards the sites of inflammation; participates in T-lymphocyte development and migration; required for B-lymphocyte development and signaling; regulates lymphocyte cytokine production; participates in neutrophil respiratory burst, chemotaxis and extravasation; promotes platelet aggregation and thrombosis; regulates alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) adhesive function in platelets downstream of P2Y12 through a lipid kinase activity-independent mechanism; involved in endothelial progenitor cell migration; modulates cardiac contractility by anchoring protein kinase A (PKA) and PDE3B activation, reducing cAMP levels; regulates cardiac contractility also by promoting beta-adrenergic receptor internalization by binding to ADRBK1 and by non-muscle tropomyosin phosphorylation; and contributes to cardiac hypertrophy under pathological stress. Possesses serine/threonine protein kinase activity: both lipid and protein kinase activities are required for beta-adrenergic receptor endocytosis. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis. Holoenzyme is a trimer composed of a heterodimer of a catalytic subunit PIK3CG and a PIK3R5 or PIK3R6 regulatory subunit. Interacts with HRAS1; the interaction is required for membrane recruitment and beta-gamma G protein dimer-dependent activation of the PI3K gamma complex PIK3CG:PIK3R6.

Protein type: Carbohydrate Metabolism - inositol phosphate; Motility/polarity/chemotaxis; EC 2.7.1.153; EC 2.7.11.1; Kinase, lipid; Autophagy

Chromosomal Location of Human Ortholog: 7q22.3

Cellular Component: mast cell granule; membrane; cytoplasm; plasma membrane; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; ephrin receptor binding; 1-phosphatidylinositol-3-kinase activity; phosphoinositide 3-kinase activity; phosphatidylinositol-4-phosphate 3-kinase activity; ATP binding; protein kinase activity

Biological Process: respiratory burst during defense response; platelet activation; neutrophil chemotaxis; phosphoinositide 3-kinase cascade; adaptive immune response; T cell activation; cytokine production; endocytosis; mast cell degranulation; protein amino acid phosphorylation; T cell proliferation; positive regulation of protein kinase B signaling cascade; G-protein coupled receptor protein signaling pathway; positive regulation of MAP kinase activity; regulation of cell adhesion mediated by integrin; elevation of cytosolic calcium ion concentration; positive regulation of acute inflammatory response; phosphoinositide phosphorylation; phospholipid metabolic process; phosphatidylinositol biosynthetic process; innate immune response; dendritic cell chemotaxis; angiogenesis; blood coagulation; inflammatory response; secretory granule localization