Rabbit PI 4-Kinase, beta Polyclonal Antibody | anti-PI4KB antibody
PI 4-Kinase, beta (AP)
Gene Names
PI4KB; NPIK; PI4K92; PIK4CB; PI4KBETA; PI4K-BETA; PI4KIIIBETA
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Western Blot, Immunoprecipitation
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PI 4-Kinase; beta; Polyclonal Antibody; beta (AP); anti-PI4KB antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes wortmannin-sensitive human PI 4-Kinase beta. Species Crossreactivity: Mouse and rat.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Applicable Applications for anti-PI4KB antibody
Immunocytochemistry (ICC), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
ICC: Reported to detect PI 4-Kinase beta in paraformaldehyde-fixed human platelets and the COS cell line.4
IP: 5ug immunoprecipitated PI 4-Kinase beta from 500ug of Jurkat RIPA lysate.
Applications are based on unconjugated antibody.
WB: 1-2ug/ml detected PI4-Kinase, beta in modified RIPA lysates of Jurkat cells. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with. Proteins were visualized using a goat anti rabbit-IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.
IP: 5ug immunoprecipitated PI 4-Kinase beta from 500ug of Jurkat RIPA lysate.
Applications are based on unconjugated antibody.
WB: 1-2ug/ml detected PI4-Kinase, beta in modified RIPA lysates of Jurkat cells. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with. Proteins were visualized using a goat anti rabbit-IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunogen
GST fusion protein corresponding to residues 410-537 of human PI 4-Kinase beta.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile (40-50%) glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Product Categories/Family for anti-PI4KB antibody
References
General 1. Enderrmann, G., et al., Biochemistry 26: 6845, 1987. 2. Pike, L., et al., Endocr. Rev. 12: 692, 1992. Application 3. Meyers, R. and Cantley, L.C., J.Biol. Chem. 272: 4384, 1997. 4. Wong, K., et al., J. Biol. Chem. 272: 13236-13241, 1997.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
91,379 Da
NCBI Official Full Name
phosphatidylinositol 4-kinase beta isoform 3
NCBI Official Synonym Full Names
phosphatidylinositol 4-kinase, catalytic, beta
NCBI Official Symbol
PI4KB
NCBI Official Synonym Symbols
NPIK; PI4K92; PIK4CB; PI4KBETA; PI4K-BETA; PI4KIIIBETA
NCBI Protein Information
phosphatidylinositol 4-kinase beta; PtdIns 4-kinase beta; type III phosphatidylinositol 4-kinase beta; phosphatidylinositol 4-kinase, wortmannin-sensitive
UniProt Protein Name
Phosphatidylinositol 4-kinase beta
Protein Family
UniProt Gene Name
PI4KB
UniProt Synonym Gene Names
PIK4CB; PI4K-beta; PI4Kbeta
UniProt Entry Name
PI4KB_HUMAN
Uniprot Description
PIK4CB: a member of the PI3/PI4 kinase family. Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol- 1,4,5,-trisphosphate. Found in the outer membrane of mitochondria and membranes of the rough endoplasmic reticulum. Recruited to the Golgi complex by the small GTPase ARF to stimulate the synthesis of phosphatidylinositol 4,5-biphosphate (PIP2) on the Golgi complex. May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Three splice-variant isoforms have been described.
Protein type: Kinase, lipid; EC 2.7.1.67; Motility/polarity/chemotaxis; Carbohydrate Metabolism - inositol phosphate
Chromosomal Location of Human Ortholog: 1q21
Cellular Component: Golgi membrane; mitochondrial outer membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; rough endoplasmic reticulum membrane; cytosol; endosome
Molecular Function: 1-phosphatidylinositol 4-kinase activity; protein binding; ATP binding
Biological Process: receptor-mediated endocytosis; phosphoinositide-mediated signaling; phosphoinositide phosphorylation; phospholipid metabolic process; phosphatidylinositol biosynthetic process; signal transduction
Research Articles on PI4KB
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Product Notes
The PI4KB pi4kb (Catalog #AAA6493498) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PI 4-Kinase, beta (AP) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PI 4-Kinase, beta can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Western Blot (WB), Immunoprecipitation (IP). ICC: Reported to detect PI 4-Kinase beta in paraformaldehyde-fixed human platelets and the COS cell line.4 IP: 5ug immunoprecipitated PI 4-Kinase beta from 500ug of Jurkat RIPA lysate. Applications are based on unconjugated antibody. WB: 1-2ug/ml detected PI4-Kinase, beta in modified RIPA lysates of Jurkat cells. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with. Proteins were visualized using a goat anti rabbit-IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Researchers should empirically determine the suitability of the PI4KB pi4kb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PI 4-Kinase, beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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