Interleukin-2 Recombinant Protein | IL2 recombinant protein

Recombinant Human Interleukin-2

Cat. Num. AAA962195

• Gene Names: IL2; IL-2; TCGF; lymphokine
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Interleukin-2; Recombinant Human Interleukin-2; T-cell growth factor; TCGF; INN: Aldesleukin; IL2 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 21-153aa; Full Length of Mature Protein
• Sequence: APTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLT
• Sequence Length: 153

• Production Note: Special Offer: The Yeast host-expressed protein is manufactured from a stock plasmid containing the protein gene. Yeasthost-expressed protein is stocked in different unit sizes ranging from as small as 10 ug to as large as 1 mg. Bulk inventory is also available. The Yeast host-expressed protein has been ordered over and over again by researchers and has stood the test of time as both a robust protein and important target for the research community. It is part of our new program to make our most popular protein targets and corresponding hosts available in expanded unit sizes and with a quick processing time. Select Yeast host-expressed protein for the fastest delivery among all hosts. Please contact our technical support team or email to [email protected] for more details.
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for IL2 recombinant protein

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.

Product Categories/Family for IL2 recombinant protein

Immunology

References

Cloning of interleukin 2 mRNAs from human tonsils.Maeda S., Nishino N., Obaru K., Mita S., Nomiyama H., Shimada K., Fujimoto K., Teranishi T., Hirano T., Onoue K.Biochem. Biophys. Res. Commun. 115:1040-1047(1983) Structure and expression of a cloned cDNA for human interleukin-2.Taniguchi T., Matsui H., Fujita T., Takaoka C., Kashima N., Yoshimoto R., Hamuro J.Nature 302:305-310(1983) Molecular cloning of human interleukin 2 cDNA and its expression in E. coli.Devos R., Plaetinck G., Cheroutre H., Simons G., Degrave W., Tavernier J., Remaut E., Fiers W.Nucleic Acids Res. 11:4307-4323(1983) Structure of the human interleukin 2 gene.Fujita T., Takaoka C., Matsui H., Taniguchi T.Proc. Natl. Acad. Sci. U.S.A. 80:7437-7441(1983) DNA sequence of the 5' flanking region of the human interleukin 2 gene homologies with adult T-cell leukemia virus.Holbrook N.J., Lieber M., Crabtree G.R.Nucleic Acids Res. 12:5005-5013(1984) T-cell growth factor complete nucleotide sequence and organization of the gene in normal and malignant cells.Holbrook N.J., Smith K.A., Fornace A.J. Jr., Comeau C.M., Wiskocil R.L., Crabtree G.R.Proc. Natl. Acad. Sci. U.S.A. 81:1634-1638(1984) Interleukin-2 transcripts in human and rodent brains possible expression by astrocytes.Eizenberg O., Faber-Elman A., Lotan M., Schwartz M.J. Neurochem. 64:1928-1936(1995) Sequence of interleukin-2 isolated from human placental poly A+ RNA possible role in maintenance of fetal allograft.Chernicky C.L., Tan H., Burfeind P., Ilan J., Ilan J.3.0.CO;2-N>Mol. Reprod. Dev. 43:180-186(1996) SeattleSNPs variation discovery resource Organization of the DNA regions flanking the human interleukin 2 gene.Nishino N., Obaru K., Maeda S., Shimada K., Onoue K.Biomed. Res. 6:197-205(1985) Promoter region of interleukin-2 gene undergoes chromatin structure changes and confers inducibility on chloramphenicol acetyltransferase gene during activation of T cells.Siebenlist U., Durand D.B., Bressler P., Holbrook N.J., Norris C.A., Kamoun M., Kant J.A., Crabtree G.R.Mol. Cell. Biol. 6:3042-3049(1986) Structure-activity relationships of recombinant human interleukin 2.Weir M.P., Chaplin M.A., Wallace D.M., Dykes C.W., Hobden A.N.Biochemistry 27:6883-6892(1988) Amino acid sequence and post-translational modification of human interleukin 2.Robb R.J., Kutny R.M., Panico M., Morris H.R., Chowdhry V.Proc. Natl. Acad. Sci. U.S.A. 81:6486-6490(1984) Expression of human interleukin-2 in recombinant baby hamster kidney, Ltk-, and Chinese hamster ovary cells. Structure of O-linked carbohydrate chains and their location within the polypeptide.Conradt H.S., Nimtz M., Dittmar K.E.J., Lindenmaier W., Hoppe J., Hauser H.J. Biol. Chem. 264:17368-17373(1989) Three-dimensional structure of interleukin-2.Brandhuber B.J., Boone T., Kenney W.C., McKay D.B.Science 238:1707-1709(1987) Unraveling the structure of IL-2.Bazan J.F.Science 257:410-412(1992) McKay D.B.Science 257:412-413(1992) Secondary structure of human interleukin 2 from 3D heteronuclear NMR experiments.Mott H.R., Driscoll P.C., Boyd J., Cooke R.M., Weir M.P., Campbell I.D.Biochemistry 31:7741-7744(1992) The interleukin-2 and interleukin-4 receptors studied by molecular modelling.Bamborough P., Hedgecock C.J., Richards W.G.Structure 2:839-851(1994) Structure of the quaternary complex of interleukin-2 with its alpha, beta, and gammac receptors.Wang X., Rickert M., Garcia K.C.Science 310:1159-1163(2005) Crystal structure of the IL-2 signaling complex paradigm for a heterotrimeric cytokine receptor.Stauber D.J., Debler E.W., Horton P.A., Smith K.A., Wilson I.A.Proc. Natl. Acad. Sci. U.S.A. 103:2788-2793(2006)

NCBI and Uniprot Product Information

• NCBI GI #: 28178861
• NCBI GeneID: 3558
• NCBI Accession #: NP_000577.2
• NCBI GenBank Nucleotide #: NM_000586.3
• UniProt Accession #: P60568; P01585
• Molecular Weight: 17.4 kDa
• NCBI Official Full Name: interleukin-2
• NCBI Official Synonym Full Names: interleukin 2
• NCBI Official Symbol: IL2
• NCBI Official Synonym Symbols: IL-2; TCGF; lymphokine
• NCBI Protein Information: interleukin-2
• UniProt Protein Name: Interleukin-2
• Protein Family: Interleukin
• UniProt Gene Name: IL2
• UniProt Synonym Gene Names: IL-2; TCGF
• UniProt Entry Name: IL2_HUMAN

NCBI Description

The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]

Uniprot Description

IL2: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells. A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. Belongs to the IL-2 family.

Protein type: Secreted, signal peptide; Secreted; Oncoprotein; Cytokine

Chromosomal Location of Human Ortholog: 4q26-q27

Cellular Component: cytosol; extracellular region; extracellular space

Molecular Function: carbohydrate binding; cytokine activity; glycosphingolipid binding; growth factor activity; interleukin-2 receptor binding; kappa-type opioid receptor binding; kinase activator activity

Biological Process: activation of MAPKK activity; adaptive immune response; axon guidance; cell adhesion; cell-cell signaling; elevation of cytosolic calcium ion concentration; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; immune response; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; natural killer cell activation; negative regulation of apoptosis; negative regulation of B cell apoptosis; negative regulation of heart contraction; negative regulation of inflammatory response; negative regulation of lymphocyte proliferation; negative regulation of protein amino acid phosphorylation; nerve growth factor receptor signaling pathway; positive regulation of activated T cell proliferation; positive regulation of B cell proliferation; positive regulation of cell growth; positive regulation of cell proliferation; positive regulation of immunoglobulin secretion; positive regulation of inflammatory response; positive regulation of interferon-gamma production; positive regulation of interleukin-17 production; positive regulation of isotype switching to IgG isotypes; positive regulation of regulatory T cell differentiation; positive regulation of tissue remodeling; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of Stat5 protein; protein kinase C activation; Ras protein signal transduction; regulation of T cell homeostatic proliferation; response to ethanol; small GTPase mediated signal transduction; T cell differentiation; vascular endothelial growth factor receptor signaling pathway