Rabbit PI 3-Kinase p85alpha Polyclonal Antibody | anti-PIK3R1 antibody

PI 3-Kinase p85alpha (Phospho Tyr607) Polyclonal Antibody

Cat. Num. AAA9701234

• Gene Names: PIK3R1; p85; AGM7; GRB1; IMD36; p85-ALPHA
• Reactivity: Human, Mouse, Rat, Chicken
• Applications: Western Blot, Immunofluorescence, Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: PI 3-Kinase p85alpha; Polyclonal Antibody; PI 3-Kinase p85alpha (Phospho Tyr607) Polyclonal Antibody; Rabbit Anti-PI 3-kinase p85alpha (phospho Tyr607) Polyclonal Antibody; PIK3R1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alph; anti-PIK3R1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Chicken
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Phospho-PI 3-kinase p85alpha (Y607) Polyclonal Antibody detects endogenous levels of PI 3-kinase p85alpha protein only when phosphorylated at Y607.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid; PBS Containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 361

• Applicable Applications for anti-PIK3R1 antibody: Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:100-1:300; ELISA: 1:10000
• Immunogen: Synthesized peptide derived from human PI 3-kinase p85alpha around the phosphorylation site of Y607
• Preparation and Storage: Stable for one year at -20 degree C from date of shipment.; For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Western Blot (WB)

(Fig.1. Western Blot analysis of various cells using Phospho-PI 3-kinase p85alpha (Y607) Polyclonal Antibody diluted at 1:1000.)

Immunohistochemistry (IHC)

(Fig.2. Immunohistochemical analysis of paraffin-embedded human liver tissue. 1, PI 3-kinase p85alpha (phospho Tyr607) Polyclonal Antibody was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)

Immunohistochemistry (IHC)

(Fig.3. Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, PI 3-kinase p85alpha (phospho Tyr607) Polyclonal Antibody was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)

Immunohistochemistry (IHC)

(Fig.4. Immunohistochemical analysis of paraffin-embedded rat heart tissue. 1, PI 3-kinase p85alpha (phospho Tyr607) Polyclonal Antibody was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)

Related Product Information for anti-PIK3R1 antibody

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. PIK3R1 encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in PIK3R1 has been associated with insulin resistance. Alternative splicing of PIK3R1 results in four transcript variants encoding different isoforms.

NCBI and Uniprot Product Information

• NCBI GI #: 335057534
• NCBI GeneID: 5295
• NCBI Accession #: NP_001229395.1
• NCBI GenBank Nucleotide #: NP_001229395.1
• UniProt Accession #: P27986
• NCBI Official Full Name: phosphatidylinositol 3-kinase regulatory subunit alpha isoform 4
• NCBI Official Synonym Full Names: phosphoinositide-3-kinase regulatory subunit 1
• NCBI Official Symbol: PIK3R1
• NCBI Official Synonym Symbols: p85; AGM7; GRB1; IMD36; p85-ALPHA
• NCBI Protein Information: phosphatidylinositol 3-kinase regulatory subunit alpha
• UniProt Protein Name: Phosphatidylinositol 3-kinase regulatory subunit alpha
• Protein Family: Phosphatidylinositol 3-kinase regulatory
• UniProt Gene Name: PIK3R1
• UniProt Synonym Gene Names: GRB1; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha
• UniProt Entry Name: P85A_HUMAN

NCBI Description

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]

Uniprot Description

PIK3R1: regulatory subunit of phosphoinositide-3-kinase. Mediates binding to a subset of tyrosine-phosphorylated proteins through its SH2 domain. Acts as an adapter, mediating the association of the p110 catalytic unit of the alpha, beta and delta enzymes to the plasma membrane, where p110 phosphorylates inositol lipids. May play an additional role in the regulation of the actin cytoskeleton. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Its SH2 domains interacts with the YTHM motif of phosphorylated INSR in vitro. Defects in PIK3R1 are a cause of severe insulin resistance. Four alternatively spliced isoforms have been described.

Protein type: Enzyme, regulatory subunit; Kinase, lipid; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q13.1

Cellular Component: membrane; cytoplasm; plasma membrane; intercellular junction; phosphoinositide 3-kinase complex; nucleus; cytosol

Molecular Function: insulin binding; insulin-like growth factor receptor binding; protein binding; ErbB-3 class receptor binding; neurotrophin TRKA receptor binding; insulin receptor substrate binding; phosphoinositide 3-kinase binding; phosphoinositide 3-kinase regulator activity; transmembrane receptor protein tyrosine kinase adaptor protein activity; transcription factor binding; protein phosphatase binding; insulin receptor binding

Biological Process: negative regulation of osteoclast differentiation; phosphoinositide 3-kinase cascade; viral reproduction; nerve growth factor receptor signaling pathway; protein amino acid phosphorylation; T cell receptor signaling pathway; positive regulation of RNA splicing; regulation of phosphoinositide 3-kinase activity; NFAT protein import into nucleus; positive regulation of glucose import; phosphoinositide phosphorylation; phospholipid metabolic process; positive regulation of transcription factor import into nucleus; epidermal growth factor receptor signaling pathway; platelet activation; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; protein stabilization; negative regulation of cell-matrix adhesion; positive regulation of tumor necrosis factor production; DNA damage response, signal transduction resulting in induction of apoptosis; insulin-like growth factor receptor signaling pathway; induction of apoptosis via death domain receptors; cellular response to insulin stimulus; B cell differentiation; T cell costimulation; insulin receptor signaling pathway; phosphatidylinositol biosynthetic process; innate immune response; cell glucose homeostasis; positive regulation of transcription from RNA polymerase II promoter; vascular endothelial growth factor receptor signaling pathway; blood coagulation; leukocyte migration; positive regulation of cell migration; negative regulation of apoptosis

Disease: Agammaglobulinemia 7, Autosomal Recessive; Short Syndrome; Immunodeficiency 36