Rabbit anti-Human PHYHD1 Polyclonal Antibody | anti-PHYHD1 antibody

PHYHD1, CT (PHYHD1, Phytanoyl-CoA dioxygenase domain-containing protein 1) (FITC)

Cat. Num. AAA6333511

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PHYHD1; Polyclonal Antibody; CT (PHYHD1; Phytanoyl-CoA dioxygenase domain-containing protein 1) (FITC); Phytanoyl-CoA dioxygenase domain-containing protein 1; anti-PHYHD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 297

• Applicable Applications for anti-PHYHD1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PHYHD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 231-260 amino acids from the C-terminal region of human PHYHD1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PHYHD1 Antibody (C-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the PHYHD1 antibody detected the PHYHD1 protein (arrow).)

Related Product Information for anti-PHYHD1 antibody

PHYHD1 probably acts as alpha-ketoglutatare-dependent dioxygenase (By similarity).

Product Categories/Family for anti-PHYHD1 antibody

Antibodies; Enzymes; Oxidase

References

Denoeud, F., et al. Genome Res. 17(6):746-759(2007). Lamesch, P., et al. Genomics 89(3):307-315(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 154937348
• NCBI GeneID: 254295
• NCBI Accession #: NP_777593.2; NP_001094347.1
• NCBI GenBank Nucleotide #: NP_777593.2
• UniProt Accession #: Q5SRE7
• NCBI Official Full Name: phytanoyl-CoA dioxygenase domain-containing protein 1 isoform b
• NCBI Official Synonym Full Names: phytanoyl-CoA dioxygenase domain containing 1
• NCBI Official Symbol: PHYHD1
• NCBI Protein Information: phytanoyl-CoA dioxygenase domain-containing protein 1
• UniProt Protein Name: Phytanoyl-CoA dioxygenase domain-containing protein 1
• Protein Family: Phytanoyl-CoA dioxygenase domain-containing protein
• UniProt Gene Name: PHYHD1

Uniprot Description

PHYHD1: Isoform 1 has alpha-ketoglutarate-dependent dioxygenase activity. Does not show detectable activity towards fatty acid CoA thioesters. Is not expected to be active with phytanoyl CoA. Isoform 2 and isoform 3 probably lack enzyme activity. Belongs to the PhyH family. PHYHD1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.-.-.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 9q34.11

Molecular Function: dioxygenase activity; metal ion binding; protein binding