Immunofluorescence (IF)
(Fluorescent image of U251 cell stained with PHOX2B Antibody (Center). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PHOX2B primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).PHOX2B immunoreactivity is localized to Nucleus significantly.)
Rabbit PHOX2B Polyclonal Antibody | anti-PHOX2B antibody
PHOX2B Antibody (Center)
WB~~1:1000
Immunofluorescence (IF)
(Fluorescent image of U251 cell stained with PHOX2B Antibody (Center). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PHOX2B primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).PHOX2B immunoreactivity is localized to Nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent image of U251 cell stained with PHOX2B Antibody (Center). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PHOX2B primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).PHOX2B immunoreactivity is localized to Nucleus significantly.)
Western Blot (WB)
(PHOX2B Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the PHOX2B antibody detected the PHOX2B protein (arrow).)
Western Blot (WB)
(PHOX2B Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the PHOX2B antibody detected the PHOX2B protein (arrow).)
member of the paired family of homeobox proteins localized to the
nucleus. The protein functions as a transcription factor involved
in the development of several major noradrenergic neuron
populations and the determination of neurotransmitter phenotype.
The gene product is linked to enhancement of second
messenger-mediated activation of the dopamine beta-hydroylase,
c-fos promoters and several enhancers, including cyclic
amp-response element and serum-response element. [provided by
RefSeq].
Janoueix-Lerosey, I., et al. Oncogene 29(11):1566-1579(2010)
Tu, E., et al. Hum. Pathol. 41(3):392-400(2010)
Arai, H., et al. J. Hum. Genet. 55(1):4-7(2010)
Dubreuil, V., et al. J. Neurosci. 29(47):14836-14846(2009)
NCBI and Uniprot Product Information
NCBI Description
The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription factor involved in the development of several major noradrenergic neuron populations and the determination of neurotransmitter phenotype. The gene product is linked to enhancement of second messenger-mediated activation of the dopamine beta-hydroylase, c-fos promoters and several enhancers, including cyclic amp-response element and serum-response element. [provided by RefSeq, Jul 2008]
Uniprot Description
PHOX2B: Involved in the development of several major noradrenergic neuron populations, including the locus coeruleus. Transcription factor which could determine a neurotransmitter phenotype in vertebrates. Enhances second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, and of several enhancers including cAMP-response element and serum- response element. Defects in PHOX2B are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. Most mutations consist of 5-10 alanine expansions in the poly-Ala region from amino acids 241-260. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. Defects in PHOX2B are the cause of susceptibility to neuroblastoma type 2 (NBLST2). A common neoplasm of early childhood arising from embryonic cells that form the primitive neural crest and give rise to the adrenal medulla and the sympathetic nervous system. Belongs to the paired homeobox family.
Protein type: Transcription regulation; DNA-binding
Chromosomal Location of Human Ortholog: 4p12
Cellular Component: nuclear chromatin
Biological Process: hindbrain tangential cell migration; autonomic nervous system development; transcription, DNA-dependent; neuron migration; neurological control of breathing; enteric nervous system development; rhombencephalic reticular formation development; negative regulation of cell proliferation; sympathetic nervous system development; cell differentiation in hindbrain; negative regulation of neuron differentiation; parasympathetic nervous system development; glial cell differentiation; regulation of gene expression; efferent axon development in a lateral line nerve; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; inner ear development
Disease: Neuroblastoma, Susceptibility To, 2; Central Hypoventilation Syndrome, Congenital
Research Articles on PHOX2B
Similar Products
Product Notes
The PHOX2B phox2b (Catalog #AAA9206242) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 104-130. The PHOX2B Antibody (Center) reacts with Mouse (Predicted Reactivity: Human, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's PHOX2B can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA), Western Blot (WB). IF~~1:10~50 WB~~1:1000. Researchers should empirically determine the suitability of the PHOX2B phox2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHOX2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
