Rabbit PHOX2B Polyclonal Antibody | anti-PHOX2B antibody

PHOX2B Antibody

Cat. Num. AAA9403893

• Gene Names: PHOX2B; PMX2B; NBLST2; NBPhox
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity chromatography purified via peptide column
• Synonyms: PHOX2B; Polyclonal Antibody; PHOX2B Antibody; Paired-like homeobox 2B; Paired mesoderm homeobox protein 2B; PMX2B; Neuroblastoma Phox; NBLST2; NBPhox; anti-PHOX2B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: PHOX2B antibody is predicted to not cross-react with other paired homeobox family members.
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 314

• Applicable Applications for anti-PHOX2B antibody: ELISA (EIA), Western Blot (WB)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 15 amino acid peptide near the center of human PHOX2B.
• Target Name: PHOX2B
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of PHOX2B in 293 cell lysate with PHOX2B antibody at (A) 1 and (B) 2 ug/mL.)

Related Product Information for anti-PHOX2B antibody

PHOX2A and PHOX2B (Paired mesoderm homeobox protein) are closely related, paired-homeodomain transcription factors that function as determinants of the noradrenergic phenotype during embryogenesis. PHOX2 proteins are crucial for the regulation of endogenous hydroxylases in neural crest cells and promote sympathetic neuron generation. Human PHOX2B contains one DNA binding homeobox domain and is required for the differentiation of all central and nonperipheral noradrenergic centers in the brain. In contrast, PHOX2A controls only the differentiation of the main noradrenergic center of the brain. Regulation of PHOX2 may have therapeutic utility in aging or disorders involving degeneration of noradrenergic neurons.

Product Categories/Family for anti-PHOX2B antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 12707580
• NCBI GeneID: 8929
• NCBI Accession #: NP_003915
• NCBI GenBank Nucleotide #: NM_003924.3
• Molecular Weight: 31,621 Da
• NCBI Official Full Name: paired mesoderm homeobox protein 2B
• NCBI Official Synonym Full Names: paired-like homeobox 2b
• NCBI Official Symbol: PHOX2B
• NCBI Official Synonym Symbols: PMX2B; NBLST2; NBPhox
• NCBI Protein Information: paired mesoderm homeobox protein 2B
• UniProt Protein Name: Paired mesoderm homeobox protein 2B
• Protein Family: Paired mesoderm homeobox protein
• UniProt Gene Name: PHOX2B
• UniProt Synonym Gene Names: PMX2B; NBPhox
• UniProt Entry Name: PHX2B_HUMAN

NCBI Description

The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription factor involved in the development of several major noradrenergic neuron populations and the determination of neurotransmitter phenotype. The gene product is linked to enhancement of second messenger-mediated activation of the dopamine beta-hydroylase, c-fos promoters and several enhancers, including cyclic amp-response element and serum-response element. [provided by RefSeq, Jul 2008]

Uniprot Description

PHOX2B: Involved in the development of several major noradrenergic neuron populations, including the locus coeruleus. Transcription factor which could determine a neurotransmitter phenotype in vertebrates. Enhances second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, and of several enhancers including cAMP-response element and serum- response element. Defects in PHOX2B are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. Most mutations consist of 5-10 alanine expansions in the poly-Ala region from amino acids 241-260. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. Defects in PHOX2B are the cause of susceptibility to neuroblastoma type 2 (NBLST2). A common neoplasm of early childhood arising from embryonic cells that form the primitive neural crest and give rise to the adrenal medulla and the sympathetic nervous system. Belongs to the paired homeobox family.

Protein type: DNA-binding; Transcription regulation

Chromosomal Location of Human Ortholog: 4p12

Cellular Component: nuclear chromatin

Biological Process: hindbrain tangential cell migration; autonomic nervous system development; transcription, DNA-dependent; neuron migration; neurological control of breathing; enteric nervous system development; rhombencephalic reticular formation development; negative regulation of cell proliferation; negative regulation of neuron differentiation; parasympathetic nervous system development; cell differentiation in hindbrain; sympathetic nervous system development; glial cell differentiation; regulation of gene expression; efferent axon development in a lateral line nerve; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; inner ear development

Disease: Neuroblastoma, Susceptibility To, 2; Central Hypoventilation Syndrome, Congenital