Western Blot (WB)
(Western Blot analysis of Phosphotyrosine on A431 cells lysates treated ( Line 1) and untreated with Sodium metavanadate using anti- Phosphotyrosine antibody at 1/100 dilution.)
Rabbit Phosphotyrosine Polyclonal Antibody
Phosphotyrosine Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
Phosphotyrosine; Polyclonal Antibody; Phosphotyrosine Polyclonal Antibody; anti-Phosphotyrosine antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Phosphotyrosine polyclonal antibody detects endogenous levels of Phosphotyrosine protein.
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Applicable Applications for anti-Phosphotyrosine antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:50-1:200
FC/FACS: 1:10-1:50
ICC: 1:50
FC/FACS: 1:10-1:50
ICC: 1:50
Immunogen
Synthetic peptide conjugated to Keyhole Limpet Haemocyanin (KLH)
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of Phosphotyrosine on A431 cells lysates treated ( Line 1) and untreated with Sodium metavanadate using anti- Phosphotyrosine antibody at 1/100 dilution.)
Western Blot (WB)
(Western Blot analysis of Phosphotyrosine on A431 cells lysates treated ( Line 1) and untreated with Sodium metavanadate using anti- Phosphotyrosine antibody at 1/100 dilution.)
Immunocytochemistry (ICC)
(ICC staining Phosphotyrosine in A431 cells (green) nontreated (Left) or treated with Sodium metavanadate (Right). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining Phosphotyrosine in A431 cells (green) nontreated (Left) or treated with Sodium metavanadate (Right). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-Phosphotyrosine antibody
Tyrosine phosphorylation plays a key role in cellular signaling. Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals. Antibodies specific for phospho-tyrosine have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.
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Product Notes
The Phosphotyrosine (Catalog #AAA3013253) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phosphotyrosine Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Phosphotyrosine can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:50-1:200 FC/FACS: 1:10-1:50 ICC: 1:50. Researchers should empirically determine the suitability of the Phosphotyrosine for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phosphotyrosine, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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