Phosphoprotein Associated With Glycosphingolipid Microdomains 1 Recombinant Protein | PAG1 recombinant protein

Recombinant Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1)

Cat. Num. AAA2029305

• Gene Names: PAG1; CBP; PAG
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Phosphoprotein Associated With Glycosphingolipid Microdomains 1; Recombinant Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1); PAG1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- IPP ENAVDAMLTA RAADGDSGPG VEGPYEVLKD SSSQENMVED CLYETVKEIK EVADKSQGGK SKSTSALKEL QGAHAEGKAD FAEYASVDRN KKCRHSTNAE SILGTSSDLD EETPPPVPVK LLDENANLPE KGEHGAEEQA PEAPSGHSKR FSSLSYKSRE EDPTLTEEEI SAMYSSVNKP GQSAHKPGPESTCQCPQGPP QRSSSSCNDL YATVKDF
• Sequence Length: 432

• Applicable Applications for PAG1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 27.4kDa
• Accurate Molecular Mass (KD): 25kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag, T7 tag
• Organism Species: Rattus norvegicus (Rat)
• Fragment: Ile138~Phe357
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 63054864
• NCBI GeneID: 55824
• NCBI Accession #: NP_060910.3
• NCBI GenBank Nucleotide #: NM_018440.3
• Molecular Weight: 46,981 Da
• NCBI Official Full Name: phosphoprotein associated with glycosphingolipid-enriched microdomains 1
• NCBI Official Synonym Full Names: phosphoprotein membrane anchor with glycosphingolipid microdomains 1
• NCBI Official Symbol: PAG1
• NCBI Official Synonym Symbols: CBP; PAG
• NCBI Protein Information: phosphoprotein associated with glycosphingolipid-enriched microdomains 1
• UniProt Protein Name: Phosphoprotein associated with glycosphingolipid-enriched microdomains 1
• Protein Family: Protein
• UniProt Gene Name: PAG1
• UniProt Synonym Gene Names: CBP; PAG
• UniProt Entry Name: PHAG1_HUMAN

NCBI Description

The protein encoded by this gene is a type III transmembrane adaptor protein that binds to the tyrosine kinase csk protein. It is thought to be involved in the regulation of T cell activation. [provided by RefSeq, Jul 2008]

Uniprot Description

PAG: an ubiquitous type III transmembrane adaptor protein that binds to the tyrosine kinase CSK protein. It is thought to be involved in the regulation of T cell activation.

Protein type: Membrane protein, integral; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 8q21.13

Cellular Component: integral to membrane; plasma membrane; lipid raft

Molecular Function: protein binding; SH3/SH2 adaptor activity; SH2 domain binding

Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of signal transduction; signal transduction; negative regulation of T cell activation; T cell receptor signaling pathway; regulation of T cell activation