Western Blot (WB)
(Western Blot; Sample: Recombinant PLCe1, Rat.)
Rabbit anti-Rat Phospholipase C Epsilon 1 (PLCe1) Polyclonal Antibody | anti-PLCe1 antibody
Polyclonal Antibody to Phospholipase C Epsilon 1 (PLCe1)
Gene Names
Plce1; Plce
Reactivity
Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Phospholipase C Epsilon 1 (PLCe1); Polyclonal Antibody; Polyclonal Antibody to Phospholipase C Epsilon 1 (PLCe1); PLCE; NPHS3; PPLC; Phosphoinositide phospholipase C-epsilon-1; Pancreas-enriched phospholipase C; 1-phosphatidylinositol 4; 5-bisphosphate phosphodiesterase epsilon-1; anti-PLCe1 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against PLCe1. It has been selected for its ability to recognize PLCe1 in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Liquid; 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
500ug/ml (varies by lot)
Sequence
Met1~Lys250
Applicable Applications for anti-PLCe1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.5-2ug/mL
IHC: 5-20ug/mL
ICC: 5-20ug/mL
IHC: 5-20ug/mL
ICC: 5-20ug/mL
Immunogen Sequence
Met1~Lys250
Cross Reactivity
Rat
Conjugation
None
Preparation and Storage
Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot; Sample: Recombinant PLCe1, Rat.)
Western Blot (WB)
(Western Blot; Sample: Recombinant PLCe1, Rat.)
Western Blot (WB)
(Western Blot; Sample: Mouse Cerebrum lysate; Primary Ab: 2ug/ml Rabbit Anti-Rat PLCe1 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot; Sample: Mouse Cerebrum lysate; Primary Ab: 2ug/ml Rabbit Anti-Rat PLCe1 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded testis tissue)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded testis tissue)
Product Categories/Family for anti-PLCe1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
255,393 Da
NCBI Official Full Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
NCBI Official Synonym Full Names
phospholipase C, epsilon 1
NCBI Official Symbol
Plce1
NCBI Official Synonym Symbols
Plce
NCBI Protein Information
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1; PLC-epsilon-1; phospholipase C epsilon; phospholipase C-epsilon-1; phosphoinositide phospholipase C-epsilon-1; phosphoinositide-specific phospholipase C epsilon-1; 1-phosphatidylinositol
UniProt Protein Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
Protein Family
UniProt Gene Name
Plce1
UniProt Synonym Gene Names
Plce; PLC-epsilon-1
UniProt Entry Name
PLCE1_RAT
Similar Products
Product Notes
The PLCe1 plce1 (Catalog #AAA2112340) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Phospholipase C Epsilon 1 (PLCe1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Phospholipase C Epsilon 1 (PLCe1) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.5-2ug/mL IHC: 5-20ug/mL ICC: 5-20ug/mL. Researchers should empirically determine the suitability of the PLCe1 plce1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1~Lys25 0. It is sometimes possible for the material contained within the vial of "Phospholipase C Epsilon 1 (PLCe1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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