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Western Blot (WB) (Western Blot analysis: Bovine cardiac preparation, treated with PKA (lane 1) or untreated (lane 2), was probed with MBS617405 (0.5ug/ml).)

Rabbit Phospholamban Polyclonal Antibody | anti-PLN antibody

Phospholamban, Phosphorylated (Ser16) (HRP)

Gene Names
PLN; PLB; CMD1P; CMH18
Reactivity
Human, Bovine
Applications
Western Blot
Purity
Purified by Immunoaffinity chromatography.
Synonyms
Phospholamban; Polyclonal Antibody; Phosphorylated (Ser16) (HRP); anti-PLN antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Bovine
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes Phospho-lamban phosphorylated (Ser16) (5-25kD). Additional non-specific bands may be detected at 75 and 100kD in some lysates. Species Crossreactivity: bovine
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-PLN antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
WB: 0.5-2ug/ml detects phosphorylated phospholamban from a bovine cardiac preparation that was incubated with. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
Immunogen
Synthetic peptide corresponding to aa14-25, RA[pS]TIEMPQQAR-C of human cardiac phospholamban with a cysteine added at the C-terminal, conjugated to KLH (Accession# NP_002658). Species sequence homology: human, mouse, rat, rabbit, porcine, canine
Conjugate
HRP
Antigen Modification
Phosphorylated
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis: Bovine cardiac preparation, treated with PKA (lane 1) or untreated (lane 2), was probed with MBS617405 (0.5ug/ml).)

Western Blot (WB) (Western Blot analysis: Bovine cardiac preparation, treated with PKA (lane 1) or untreated (lane 2), was probed with MBS617405 (0.5ug/ml).)
Product Categories/Family for anti-PLN antibody
References
1. Most, P., et al., J. Biol. Chem. 278: 33, 809-33, 817 (2003). 2. Mayer, E.J., et al., Biochem. Biophys. Research Comm. 267: 40-48 (2000).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
6,109 Da
NCBI Official Full Name
cardiac phospholamban
NCBI Official Synonym Full Names
phospholamban
NCBI Official Symbol
PLN
NCBI Official Synonym Symbols
PLB; CMD1P; CMH18
NCBI Protein Information
cardiac phospholamban
UniProt Protein Name
Cardiac phospholamban
Protein Family
UniProt Gene Name
PLN
UniProt Synonym Gene Names
PLB; PLB
UniProt Entry Name
PPLA_HUMAN

Uniprot Description

PLB: a heart protein postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum. A major substrate for the cAMP-dependent protein kinase. An inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. A key regulator of cardiac diastolic function. Mutations are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure.

Protein type: Membrane protein, integral; Inhibitor

Chromosomal Location of Human Ortholog: 6q22.1

Cellular Component: sarcoplasmic reticulum membrane; mitochondrion; membrane; endoplasmic reticulum; perinuclear region of cytoplasm; mitochondrial membrane; vesicle

Molecular Function: enzyme inhibitor activity; identical protein binding; protein binding; ATPase inhibitor activity; calcium channel regulator activity; ATPase binding

Biological Process: cardiac muscle development; negative regulation of calcium ion transport; negative regulation of heart rate; cytosolic calcium ion homeostasis; blood circulation; regulation of calcium ion transport; response to testosterone stimulus; regulation of heart contraction; response to insulin stimulus; response to zinc ion; calcium ion transport; negative regulation of catalytic activity; negative regulation of ATPase activity; regulation of the force of heart contraction; protein homooligomerization

Disease: Cardiomyopathy, Dilated, 1p; Cardiomyopathy, Familial Hypertrophic, 18

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Product Notes

The PLN pln (Catalog #AAA6493258) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospholamban, Phosphorylated (Ser16) (HRP) reacts with Human, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's Phospholamban can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. WB: 0.5-2ug/ml detects phosphorylated phospholamban from a bovine cardiac preparation that was incubated with. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Researchers should empirically determine the suitability of the PLN pln for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospholamban, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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