Rabbit anti-Human, Rat Phosphoinositide 3 Kinase, p110 alpha Polyclonal Antibody | anti-PK3CA antibody

Phosphoinositide 3 Kinase, p110 alpha (PI3K, caPI3K, MGC142161, MGC142163, p110 alpha, p110-alpha, Phosphatidylinositol 3 Kinase Catalytic alpha Polypeptide, Phosphatidylinositol 3 Kinase Catalytic 110kD alpha, Phosphatidylinositol-4,5-bisphosphate 3-kina

Cat. Num. AAA6493158

• Gene Names: PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; PI3K-alpha; p110-alpha
• Reactivity: Human, Rat
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Phosphoinositide 3 Kinase; p110 alpha; Polyclonal Antibody; p110 alpha (PI3K; caPI3K; MGC142161; MGC142163; p110-alpha; Phosphatidylinositol 3 Kinase Catalytic alpha Polypeptide; Phosphatidylinositol 3 Kinase Catalytic 110kD alpha; Phosphatidylinositol-4; 5-bisphosphate 3-kina; anti-PK3CA antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PI3 Kinase p110 alpha. Species Crossreactivity: rat
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PK3CA antibody: ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: WB: 1:2000 detects PI3K p110a in Jurkat cell extract.; Applications are based on unconjugated antibody.
• Immunogen: Synthetic linear peptide corresponding to an internal region of class I catalytic domain of PI3 Kinase that is specific to the p110 alpha isoform, conjugated to KLH (NP_006209.2).
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

ELISA (EIA)

(Specificity Testing (ELISA): Peptides to various PI3 Kinase, p110 isoforms including a,b,g were tested using ELISA to determine antibody specificity to the p110a isoform of PI3 Kinase)

Immunohistochemistry (IHC)

(PI3 Kinase, p110a staining of human kidney 2mm array spot. Tissue pre-treated with Citrate pH 6.0 antigen-retrieval. Polyclonal Ab (P4073-26K) diluted to 1:100, IHC-Select Detection with HRP-DAB. Immunoreactivity is seeing as a staining to include the thin and distal tubules but not the glomeruli.)

Immunoprecipitation (IP)

(Immunoprecipitation: PI3 Kinase, p110a was immunoprecipitated from 500ul (1mg/1ml) of Jurkat cell lysate using 10ug of P4073-26K. 5ul of this reaction was resolved via SDS-PAGE, transferred to Immobilon-P membrane, and probed with P4073-26K.)

Western Blot (WB)

(Western Blot Analysis: Representative lot data. Jurkat cell lysate was resolved by SDS-PAGE, transferred to PVDF membrane, and probed with P4073-26K (0.05ug/ml).)

Product Categories/Family for anti-PK3CA antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 54792082
• NCBI GeneID: 5290
• NCBI Accession #: NP_006209.2
• NCBI GenBank Nucleotide #: NM_006218.3
• UniProt Accession #: P42336; Q14CW1; Q99762
• Molecular Weight: 124,284 Da
• NCBI Official Full Name: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
• NCBI Official Synonym Full Names: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
• NCBI Official Symbol: PIK3CA
• NCBI Official Synonym Symbols: MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; PI3K-alpha; p110-alpha
• NCBI Protein Information: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
• UniProt Protein Name: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
• UniProt Gene Name: PIK3CA
• UniProt Synonym Gene Names: PI3-kinase subunit alpha; PI3K-alpha; PI3Kalpha; PtdIns-3-kinase subunit alpha; PtdIns-3-kinase subunit p110-alpha; p110alpha
• UniProt Entry Name: PK3CA_HUMAN

NCBI Description

Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22. [provided by RefSeq, Apr 2016]

Uniprot Description

PIK3CA: Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4- phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5- bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation in breast cancer cells through the PDPK1- AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. Has also serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS. Heterodimer of a catalytic subunit PIK3CA and a p85 regulatory subunit (PIK3R1, PIK3R2 or PIK3R3). Interacts with IRS1 in nuclear extracts. Interacts with RUFY3. Interacts with RASD2. Interacts with APPL1. Interacts with HRAS1 and KRAS. Interaction with HRAS1/KRAS is required for PI3K pathway signaling and cell proliferation stimulated by EGF and FGF2. Belongs to the PI3/PI4-kinase family.

Protein type: Motility/polarity/chemotaxis; Oncoprotein; EC 2.7.11.1; EC 2.7.1.153; Kinase, lipid; Carbohydrate Metabolism - inositol phosphate

Chromosomal Location of Human Ortholog: 3q26.3

Cellular Component: lamellipodium; plasma membrane; phosphoinositide 3-kinase complex; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; insulin receptor substrate binding; 1-phosphatidylinositol-3-kinase activity; kinase activity; protein kinase activator activity; ATP binding; phosphatidylinositol-4-phosphate 3-kinase activity; phosphoinositide 3-kinase activity

Biological Process: epidermal growth factor receptor signaling pathway; platelet activation; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; regulation of multicellular organism growth; glucose metabolic process; positive regulation of peptidyl-serine phosphorylation; T cell receptor signaling pathway; protein amino acid phosphorylation; vasculature development; phosphoinositide phosphorylation; positive regulation of protein kinase activity; phospholipid metabolic process; T cell costimulation; phosphatidylinositol biosynthetic process; protein kinase B signaling cascade; insulin receptor signaling pathway; innate immune response; endothelial cell migration; negative regulation of neuron apoptosis; angiogenesis; blood coagulation; vascular endothelial growth factor receptor signaling pathway; leukocyte migration; cardiac muscle contraction

Disease: Cowden Syndrome 5; Gastric Cancer; Keratosis, Seborrheic; Breast Cancer; Lung Cancer; Ovarian Cancer; Congenital Lipomatous Overgrowth, Vascular Malformations, And Epidermal Nevi; Colorectal Cancer; Megalencephaly-capillary Malformation-polymicrogyria Syndrome; Hepatocellular Carcinoma; Nevus, Epidermal