Goat Phosphoglucomutase, beta Polyclonal Antibody
Anti - Phosphoglucomutase, beta (Biotin) (PGM)
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified from monospecific antiserum by salt fractionation and ion exchange chromatography.
Synonyms
Phosphoglucomutase; beta; Polyclonal Antibody; Anti - Phosphoglucomutase; beta (Biotin) (PGM); anti-Phosphoglucomutase; beta antibody
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes the 34kD enzyme. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum as well as purified and partially purified b-Phosphoglucomutase.
Purity/Purification
Purified from monospecific antiserum by salt fractionation and ion exchange chromatography.
Form/Format
Highly Purified
Supplied as a liquid in PBS, 10mg/ml BSA (IgG and Protease free), pH 7.2, 0.01% sodium azide. Conjugated to Biotin
Supplied as a liquid in PBS, 10mg/ml BSA (IgG and Protease free), pH 7.2, 0.01% sodium azide. Conjugated to Biotin
Concentration
~10mg/ml (varies by lot)
Applicable Applications for anti-Phosphoglucomutase, beta antibody
ELISA, Western Blot, Immunohistochemistry
Application Notes
Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates
Dilution: ELISA: 1:3,000 to 1:20,000. Against 1ug of b-Phosphoglucomutase [Lactococcus lacti] in a standard sandwich ELISA using Peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at RT.
Optimal dilutions to be determined by the researcher.
Dilution: ELISA: 1:3,000 to 1:20,000. Against 1ug of b-Phosphoglucomutase [Lactococcus lacti] in a standard sandwich ELISA using Peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at RT.
Optimal dilutions to be determined by the researcher.
Immunogen
Beta Phosphoglucomutase [Lactococcus lacti]
Biotin-Protein Ratio
10-20 BAC molecules per goat IgG molecule
Label
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Phosphoglucomutase, beta antibody
Phosphoglucomutase (PGM) interconverts the 1- and 6-phosphate isomers of alpha-D-glucose. Although it is a reversible reaction, the formation of glucose-6-phosphate is markedly favored. PGM will not convert beta-D-glucose-1-phosphate or beta-D-glucose-6-phosphate.
Product Categories/Family for anti-Phosphoglucomutase, beta antibody
Similar Products
Product Notes
The Phosphoglucomutase, beta (Catalog #AAA612593) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Phosphoglucomutase, beta can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunohistochemistry. Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates Dilution: ELISA: 1:3,000 to 1:20,000. Against 1ug of b-Phosphoglucomutase [Lactococcus lacti] in a standard sandwich ELISA using Peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at RT. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Phosphoglucomutase, beta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phosphoglucomutase, beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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