Western Blot (WB)
(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human 293T Cells; Lane3: Porcine Liver Tissue; Lane4: Porcine Heart Tissue.)
Rabbit anti-Human Phosphoglucomutase 1 Polyclonal Antibody | anti-PGM1 antibody
Phosphoglucomutase 1 (PGM1) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human 293T Cells; Lane3: Porcine Liver Tissue; Lane4: Porcine Heart Tissue.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human 293T Cells; Lane3: Porcine Liver Tissue; Lane4: Porcine Heart Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant PGM1, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant PGM1, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Stomach Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Stomach Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Brain Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Brain Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue)
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: PGM1 knockout Hela cell lysate; Predicted MW: 64,61,40kd Observed MW: 64kd Primary Ab: 1ug/ml Rabbit Anti-Human PGM1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: PGM1 knockout Hela cell lysate; Predicted MW: 64,61,40kd Observed MW: 64kd Primary Ab: 1ug/ml Rabbit Anti-Human PGM1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an isozyme of phosphoglucomutase (PGM) and belongs to the phosphohexose mutase family. There are several PGM isozymes, which are encoded by different genes and catalyze the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, this PGM isozyme is predominant, representing about 90% of total PGM activity. In red cells, PGM2 is a major isozyme. This gene is highly polymorphic. Mutations in this gene cause glycogen storage disease type 14. Alternativley spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Mar 2010]
Uniprot Description
This enzyme participates in both the breakdown and synthesis of glucose.
Research Articles on PGM1
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Product Notes
The PGM1 pgm1 (Catalog #AAA2032851) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phosphoglucomutase 1 (PGM1) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Phosphoglucomutase 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the PGM1 pgm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phosphoglucomutase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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