Western Blot (WB)
(WB of recombinant GFP-PDE5A1 and phospho-PDE5A antibody. Overexpressing cells were treated with or without ANP. 1:500 in antibody dilution in DiluObuffer. Blot was stripped with stripObuffer and reprobed)
Rabbit Phospho-PDE5A Polyclonal Antibody | anti-PDE5A antibody
Phospho PDE5A Antibody FITC-Conjugated
Gene Names
PDE5A; CN5A; PDE5; CGB-PDE
Reactivity
Cross-Reactivity: Bovine, Human, Mouse, Pig, Rat, Sheep
Applications
ELISA, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Synonyms
Phospho-PDE5A; Polyclonal Antibody; Phospho PDE5A Antibody FITC-Conjugated; 5''-cyclic phosphodiesterase; CGB PDE; cGMP binding cGMP specific 3' 5' cyclic nucleotide phosphodiesterase; cGMP binding/cGMP specific phosphodiesterase; cGMP specific 3'5' cyclic phosphodiesterase; cGMP specific phosphodiesterase PDE5A2; cGMP specific phosphodiesterase type 5A; cGMP-binding cGMP-specific phosphodiesterase; cGMP-specific 3''; CN5A; CN5N; PDE 5; PDE 5A; PDE5A1; Phosphodiesterase 5A; Phosphodiesterase 5A cGMP specific; Phosphodiesterase isozyme 5 antibody; anti-PDE5A antibody
Host
Rabbit
Reactivity
Cross-Reactivity: Bovine, Human, Mouse, Pig, Rat, Sheep
Clonality
Polyclonal
Specificity
This antibody will detect only PDE5A protein that has been phosphorylated at the PKG site. Will not detect unphosphorylated PDE5A, or other PDE family members.
Form/Format
FITC-Conjugated
Sequence Length
921
Applicable Applications for anti-PDE5A antibody
ELISA (EIA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Confocal Microscopy||1:100
Dot Blot: 1:10,000
ELISA: 1:10,000
Immunocytochemistry: 1:100
Immunofluorescence: 1:100
Immunohistochemistry: 1:100
Immunoprecipitation: 1:200
Western Blot: 1:500
Dot Blot: 1:10,000
ELISA: 1:10,000
Immunocytochemistry: 1:100
Immunofluorescence: 1:100
Immunohistochemistry: 1:100
Immunoprecipitation: 1:200
Western Blot: 1:500
Immunogen
Phosphorylated synthetic peptide common to all PDE5A variants taken within amino acid region 120-170 on human PDE5A protein.
Preparation and Storage
Store at -20 degree C for long term storage
Western Blot (WB)
(WB of recombinant GFP-PDE5A1 and phospho-PDE5A antibody. Overexpressing cells were treated with or without ANP. 1:500 in antibody dilution in DiluObuffer. Blot was stripped with stripObuffer and reprobed)
Western Blot (WB)
(WB of recombinant GFP-PDE5A1 and phospho-PDE5A antibody. Overexpressing cells were treated with or without ANP. 1:500 in antibody dilution in DiluObuffer. Blot was stripped with stripObuffer and reprobed)
Related Product Information for anti-PDE5A antibody
FITC-Conjugated Phosphorylated Phosphodiesterase 5A Antibody
The cyclic monophosphate nucleotides, cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) are found ubiquitously in mammalian cells and act as second messenger transducers to effect the intracellular actions of a variety of G-protein coupled receptors (GPCRs) for hormones, cytokines, and neurotransmitters. Cyclic nucleotides are important intracellular second messengers which play important role in a variety of signal transduction process. The cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. One of the many phosphodiesterases that compartmentalized and hydrolyze cGMP in various tissues is phosphodiesterase type 5A (POE5A). The cGMP is involved in nitric oxide signaling as well as cell signaling associated with natriuretic peptides and gulanylins. Some of the intracellular biding cites for the cGMP include cyclic nucleotide gated ion channels, cGMP-dependent protein kinases, and cyclic GMP biding phosphodiesterases (cGB-POEs). The cGB-POEs include POE2, POE3, POE5 and POE10; the members of these families contain various structural and functional motifs that are conserved. Most of these proteins contain dimmeric subunits that contain a highly conserved cGMP binding site and a phosphodiesterase catalytic site. The cGMP-specific phosphodiesterase type-5A (POE5A) family is comprised of single gene with multiple splice variants (POE5A1 and POE5A2) generated by RNA splicing and use the of alternate initiation sites (1,2). POE5 is highly expressed in aorta, lungs, intestine, kidney adrenal gland, cerebellum, and cerebrum. In cerebellum, the POE5 is highly expressed during neonatal development in Purkinje cells layer. POE5 is also abundant in vascular smooth muscle regulating cGMP levels and vascular smooth muscle tonicity. In corpus cavernosum inhibition of POE5 by sildenafil corrects erectile dysfunctions. The nitric oxide donor sodium notropurside (SNP) stimulate POE5 activity by cGMP-dependent kinase phosphorylation (Rybalkin et. aI. , 2002; Murthy 2001). POE5Al has 875 amino acids (99.5 kDa). The amino terminal 142 amino acid of the POE5 gene showed no sequence homology with other POEs and also contained serine 92 that is phosphorylated by cGMP-kinases (MCAllister et aI. , 93). A phospho-specific POE5A antibody was generated against the conserved PKG phosphorylation site.
The affinity purified antibody labels POE5A proteins when they are phosphorylated at Ser92 by PKG. The POE5-selective antibody was generated using cyclic peptide methodology that results in higher titer and specificity (3). The POE5-selective antibodies are generated against peptides from unique sequences on the POE5A gene. The affinity purified antibody labels 99 and 89 kDa POE5A 1 and POE5A2 proteins using POE5A WB positive controls. The POE5A-specific antiserum has no cross reactivity against POE5B protein or any other member of the fast growing family of POEs.
The cyclic monophosphate nucleotides, cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) are found ubiquitously in mammalian cells and act as second messenger transducers to effect the intracellular actions of a variety of G-protein coupled receptors (GPCRs) for hormones, cytokines, and neurotransmitters. Cyclic nucleotides are important intracellular second messengers which play important role in a variety of signal transduction process. The cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. One of the many phosphodiesterases that compartmentalized and hydrolyze cGMP in various tissues is phosphodiesterase type 5A (POE5A). The cGMP is involved in nitric oxide signaling as well as cell signaling associated with natriuretic peptides and gulanylins. Some of the intracellular biding cites for the cGMP include cyclic nucleotide gated ion channels, cGMP-dependent protein kinases, and cyclic GMP biding phosphodiesterases (cGB-POEs). The cGB-POEs include POE2, POE3, POE5 and POE10; the members of these families contain various structural and functional motifs that are conserved. Most of these proteins contain dimmeric subunits that contain a highly conserved cGMP binding site and a phosphodiesterase catalytic site. The cGMP-specific phosphodiesterase type-5A (POE5A) family is comprised of single gene with multiple splice variants (POE5A1 and POE5A2) generated by RNA splicing and use the of alternate initiation sites (1,2). POE5 is highly expressed in aorta, lungs, intestine, kidney adrenal gland, cerebellum, and cerebrum. In cerebellum, the POE5 is highly expressed during neonatal development in Purkinje cells layer. POE5 is also abundant in vascular smooth muscle regulating cGMP levels and vascular smooth muscle tonicity. In corpus cavernosum inhibition of POE5 by sildenafil corrects erectile dysfunctions. The nitric oxide donor sodium notropurside (SNP) stimulate POE5 activity by cGMP-dependent kinase phosphorylation (Rybalkin et. aI. , 2002; Murthy 2001). POE5Al has 875 amino acids (99.5 kDa). The amino terminal 142 amino acid of the POE5 gene showed no sequence homology with other POEs and also contained serine 92 that is phosphorylated by cGMP-kinases (MCAllister et aI. , 93). A phospho-specific POE5A antibody was generated against the conserved PKG phosphorylation site.
The affinity purified antibody labels POE5A proteins when they are phosphorylated at Ser92 by PKG. The POE5-selective antibody was generated using cyclic peptide methodology that results in higher titer and specificity (3). The POE5-selective antibodies are generated against peptides from unique sequences on the POE5A gene. The affinity purified antibody labels 99 and 89 kDa POE5A 1 and POE5A2 proteins using POE5A WB positive controls. The POE5A-specific antiserum has no cross reactivity against POE5B protein or any other member of the fast growing family of POEs.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
phosphodiesterase 5A, cGMP-specific, isoform CRA_c
NCBI Official Synonym Full Names
phosphodiesterase 5A
NCBI Official Symbol
PDE5A
NCBI Official Synonym Symbols
CN5A; PDE5; CGB-PDE
NCBI Protein Information
cGMP-specific 3',5'-cyclic phosphodiesterase
UniProt Protein Name
cGMP-specific 3',5'-cyclic phosphodiesterase
UniProt Gene Name
PDE5A
UniProt Synonym Gene Names
PDE5; CGB-PDE
UniProt Entry Name
PDE5A_HUMAN
NCBI Description
This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
PDE5A: a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Phosphorylation is regulated by binding of cGMP to the two allosteric sites. Sildenafil (Viagra) is a highly selective and potent inhibitor of PDE5A. Alternative splicing results in four transcript variants encoding distinct isoforms.
Protein type: Nucleotide Metabolism - purine; Phosphodiesterase; EC 3.1.4.35
Chromosomal Location of Human Ortholog: 4q27
Cellular Component: cytosol
Molecular Function: 3',5'-cyclic-GMP phosphodiesterase activity; 3',5'-cyclic-nucleotide phosphodiesterase activity; cGMP binding; metal ion binding
Biological Process: cGMP catabolic process; negative regulation of T cell proliferation; positive regulation of MAP kinase activity; regulation of cGMP metabolic process; signal transduction
Research Articles on PDE5A
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Product Notes
The PDE5A pde5a (Catalog #AAA544220) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho PDE5A Antibody FITC-Conjugated reacts with Cross-Reactivity: Bovine, Human, Mouse, Pig, Rat, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-PDE5A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). Confocal Microscopy||1:100 Dot Blot: 1:10,000 ELISA: 1:10,000 Immunocytochemistry: 1:100 Immunofluorescence: 1:100 Immunohistochemistry: 1:100 Immunoprecipitation: 1:200 Western Blot: 1:500. Researchers should empirically determine the suitability of the PDE5A pde5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-PDE5A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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