Rabbit Phospho-PDE4D Polyclonal Antibody | anti-PDE4D antibody
Phospho PDE4D ERK site Antibody
Gene Names
PDE4D; DPDE3; PDE43; STRK1; ACRDYS2; HSPDE4D; PDE4DN2
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Synonyms
Phospho-PDE4D; Polyclonal Antibody; Phospho PDE4D ERK site Antibody; cAMP specific 3'; 5' cyclic phosphodiesterase 4D; DKFZp686M11213; 5''-cyclic phosphodiesterase 4D; cAMP specific 3' 5' cyclic phosphodiesterase 4D; cAMP specific phosphodiesterase 4D; cAMP specific phosphodiesterase PDE4D; cAMP specific phosphodiesterase PDE4D6; cAMP-specific 3''; cyclic AMP phosphodiesterase; DPDE3; Dunce like phosphodiesterase E3; FLJ97311; HSPDE4D; PDE43; PDE4DN2; Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3); Phosphodiesterase 4D cAMP specific (phosphodiesterase E3 dunce homolog Drosophila); Phosphodiesterase 4D cAMP specific; Phosphodiesterase E3 dunce homolog Drosophila; STRK 1 antibody; anti-PDE4D antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Detects endogenous levels of PDE4D only when phosphorylated at Serine 190.
Form/Format
Affinity Purified Immunoglobulins
Concentration
0.58mg/ml IgG in antibody stabilization buffer (varies by lot)
Sequence Length
687
Applicable Applications for anti-PDE4D antibody
ELISA (EIA), Immunohistochemistry - Paraffin (IHC-P), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Dot Blot: 1:10,000
ELISA: 1:10,000
Immunoprecipitation: 1:150
Western Blot: 1:500
Immunohistochemistry: 1:50-1:100
ELISA: 1:10,000
Immunoprecipitation: 1:150
Western Blot: 1:500
Immunohistochemistry: 1:50-1:100
Immunogen
Phosphorylated synthetic peptide selective for PDE4D ERK site taken within amino acid ergion 550-600 on human PDE4D8 protein.
Expression
Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
Modification
Phosphorylated
Molecular Function
Hydrolase, 3', 5'-cyclic-AMP phosphodiesterase activity, cAMP binding
Structure
Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1 (PP1).
Subcellular Location
Apical cell membrane, Cytoplasm, Membrane
Related Products
Phospho-PDE4D ERK site Antibod: MBS540516
Phospho-PDE4D PKA site Antibody: MBS540103
FITC-Conjugated: MBS544187
BIOTIN-Conjugated: MBS544188
Antigenic Blocking Peptide: MBS544314
Western Blot Positive Control: MBS543155
Phospho-PDE4D PKA site Antibody: MBS540103
FITC-Conjugated: MBS544187
BIOTIN-Conjugated: MBS544188
Antigenic Blocking Peptide: MBS544314
Western Blot Positive Control: MBS543155
Preparation and Storage
-20 degree C for long term storage
Related Product Information for anti-PDE4D antibody
Enzymes of the cAMP-dependent phosphodiesterase type 4 (PDE4) family are important in hydrolyzing cAMP produced by G-protein coupled receptor (GPCR) stimulated adenylyl cyclases. In brain more than 90% of cAMP formed by the stimulation of GPCRs is hydrolyzed by PDE4 enzymes (1). Members of the PDE4A, Band D family are associated with GPCRs (adrenergic and dopaminergic) signaling (2, 3). PDE4 enzymes are also important molecular targets for variety of therapeutic agents like antidepressants, antiasthmatics,and anti-inflammatory drugs. PDE4 family comprised of 4 genes (PDE4A, B, C and D); each exhibiting multiple isozymes due to alternate splicing that leads to a larger number of distinct PDE4 variants (4). Members of the PDE4 family are regulated/activated by phosphorylation/dephosphorylation by cAMP-dependent protein kinase A and phosphatases (5). Two conserved phosphorylation motifs have been identified in PDE4B and PDE4D. Phosphorylation at PKA site resulted in significant increase in enzymatic activity of PDE4D variants. Phosphorylation state, protein-protein interactions and cellular trafficking of PDE4D enzymes play an important role in cAMpi compartmentalization and cAMPdependent signaling (6).
Phospho-PDE4D selective antibody was generated from a peptide that represents a putative ERK phosphorylation site and is common in all PDE4D enzymes (5). To detect the non-phosphorylated PDE4D protein a PDE4D-selective antibody (MBS540072) is also available in affinity purified form. AntigeniC blocking peptide and western blot positive controls for phosphorylated and non-phosphorylated PDE4D are available for easy identification and quantification of these proteins. Antibodies can be conjugated to fluorescent probes or secondary enzymes upon request at an additional charge.
Phospho-PDE4D selective antibody was generated from a peptide that represents a putative ERK phosphorylation site and is common in all PDE4D enzymes (5). To detect the non-phosphorylated PDE4D protein a PDE4D-selective antibody (MBS540072) is also available in affinity purified form. AntigeniC blocking peptide and western blot positive controls for phosphorylated and non-phosphorylated PDE4D are available for easy identification and quantification of these proteins. Antibodies can be conjugated to fluorescent probes or secondary enzymes upon request at an additional charge.
Product Categories/Family for anti-PDE4D antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
cAMP-specific 3',5'-cyclic phosphodiesterase 4D isoform PDE4D8
NCBI Official Synonym Full Names
phosphodiesterase 4D
NCBI Official Symbol
PDE4D
NCBI Official Synonym Symbols
DPDE3; PDE43; STRK1; ACRDYS2; HSPDE4D; PDE4DN2
NCBI Protein Information
cAMP-specific 3',5'-cyclic phosphodiesterase 4D
UniProt Protein Name
cAMP-specific 3',5'-cyclic phosphodiesterase 4D
Protein Family
UniProt Gene Name
PDE4D
UniProt Synonym Gene Names
DPDE3
UniProt Entry Name
PDE4D_HUMAN
NCBI Description
This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.[provided by RefSeq, Sep 2009]
Uniprot Description
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
Research Articles on PDE4D
Similar Products
Product Notes
The PDE4D pde4d (Catalog #AAA540516) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho PDE4D ERK site Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-PDE4D can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry - Paraffin (IHC-P), Immunoprecipitation (IP), Western Blot (WB). Dot Blot: 1:10,000 ELISA: 1:10,000 Immunoprecipitation: 1:150 Western Blot: 1:500 Immunohistochemistry: 1:50-1:100. Researchers should empirically determine the suitability of the PDE4D pde4d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-PDE4D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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