Western Blot (WB)
Rabbit PDE4D5 Polyclonal Antibody | anti-PDE4D5 antibody
PDE4D5 Antibody
Gene Names
PDE4D; DPDE3; PDE43; STRK1; ACRDYS2; HSPDE4D; PDE4DN2
Reactivity
Human, Mouse, Rabbit, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Synonyms
PDE4D5; Polyclonal Antibody; PDE4D5 Antibody; cAMP-specific 3'5'-cyclic phosphodiesterase 4D; DPDE3; dunce-like phosphodiesterase E3; hPDE4D5; PDE4D; PDE4DN2; Phosphodiesterase 4D; Phosphodiesterase 4D cAMP-specific (dunce (Drosophila)-homolog phosphodiesterase E3); Phosphodiesterase 4D cAMP-specific; STRK1 antibody; anti-PDE4D5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rabbit, Rat
Clonality
Polyclonal
Specificity
This antibody does not cross-react with other members of the PDE4D family or with other PDE4 proteins (PDE4A, PDE4B and PDE4C) or with other PDE family members.
Form/Format
Affinity Purified
Concentration
0.65 ug/ul in antibody stabilization buffer (varies by lot)
Sequence Length
745
Applicable Applications for anti-PDE4D5 antibody
Confocal Microscopy (CM), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Confocal Microscopy||1:200
Dot Blot: 1:10,000
ELISA: 1:10,000
Immunocytochemistry: 1:200
Immunofluorescence: 1:200
Immunohistochemistry: 1:200
Immunoprecipitation: 1:150
Western Blot: 1:500
Dot Blot: 1:10,000
ELISA: 1:10,000
Immunocytochemistry: 1:200
Immunofluorescence: 1:200
Immunohistochemistry: 1:200
Immunoprecipitation: 1:150
Western Blot: 1:500
Immunogen
A 20 amino acid synthetic peptide taken from the N-terminus corresopnding to the PDE4D5 variant.
Expression
Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
Molecular Function
Hydrolase, 3', 5'-cyclic-AMP phosphodiesterase activity, cAMP binding
Structure
Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphotase 1 (PP1) (By similarity). Isoform 5, isoform N3 and isoform 12 bind GNB2L1 via their unique N-terminus. Binds ARRB2. Interacts (via N-terminal region) with SHANK2 (via proline-rich region); the interaction is increased in a PKA-dependent manner.
Subcellular Location
Apical cell membrane, Cytoplasm, Membrane
Preparation and Storage
-20 degree C for long term storage
Western Blot (WB)
Western Blot (WB)
Related Product Information for anti-PDE4D5 antibody
Affinity Purified cAMP-specific phosphodiesterase PDE4D5 Antibody
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
Product Categories/Family for anti-PDE4D5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
24,429 Da
NCBI Official Full Name
cAMP-specific phosphodiesterase PDE4D5
NCBI Official Synonym Full Names
phosphodiesterase 4D
NCBI Official Symbol
PDE4D
NCBI Official Synonym Symbols
DPDE3; PDE43; STRK1; ACRDYS2; HSPDE4D; PDE4DN2
NCBI Protein Information
cAMP-specific 3',5'-cyclic phosphodiesterase 4D
UniProt Protein Name
cAMP-specific 3',5'-cyclic phosphodiesterase 4D
UniProt Gene Name
PDE4D
UniProt Synonym Gene Names
DPDE3
UniProt Entry Name
PDE4D_HUMAN
NCBI Description
This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.[provided by RefSeq, Sep 2009]
Uniprot Description
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
Research Articles on PDE4D5
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Product Notes
The PDE4D5 pde4d (Catalog #AAA543529) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PDE4D5 Antibody reacts with Human, Mouse, Rabbit, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PDE4D5 can be used in a range of immunoassay formats including, but not limited to, Confocal Microscopy (CM), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). Confocal Microscopy||1:200 Dot Blot: 1:10,000 ELISA: 1:10,000 Immunocytochemistry: 1:200 Immunofluorescence: 1:200 Immunohistochemistry: 1:200 Immunoprecipitation: 1:150 Western Blot: 1:500. Researchers should empirically determine the suitability of the PDE4D5 pde4d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDE4D5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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