Western Blot
Rabbit Phospho-p27 KIP1 Polyclonal Antibody | anti-p27 KIP1 antibody
Phospho p27 KIP1 Antibody Affinity Purified
Gene Names
CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1
Reactivity
Chicken, Human, Monkey, Mouse, Rat
Applications
ELISA, Western Blot
Synonyms
Phospho-p27 KIP1; Polyclonal Antibody; Phospho p27 KIP1 Antibody Affinity Purified; AA408329; AI843786; CDKN 1B; CDKN 4; Cyclin Dependent Kinase Inhibitor 1B; Cyclin dependent kinase inhibitor p27; KIP 1; MEN1B; MEN4; p27; p27 Kip1; P27-like cyclin-dependent kinase inhibitor antibody; anti-p27 KIP1 antibody
Host
Rabbit
Reactivity
Chicken, Human, Monkey, Mouse, Rat
Clonality
Polyclonal
Form/Format
Affinity Purified
Concentration
0.50-0.75 ug/ul in antibody stabilization buffer (varies by lot)
Sequence Length
198
Applicable Applications for anti-p27 KIP1 antibody
ELISA (EIA), IMM, Western Blot (WB)
Application Notes
Dot Blot: 1:10,000
ELISA: 1:10,000
Immunoprecipitation: 1:150
Western Blot: 1:250
ELISA: 1:10,000
Immunoprecipitation: 1:150
Western Blot: 1:250
Immunogen
Synthetic peptide taken within amino acid region 143-193 on human Cyclin-dependent kinase inhibitor 1 (p27, KIP1) protein.
Expression
Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.
Modification
Phosphorylated
Determinant
C-epitope
Molecular Function
Cyclin-dependent protein serine/threonine kinase inhibitor activity, kinase activity
Structure
Forms a ternary complex with CCNE1/CDK2/CDKN1B. Interacts directly with CCNE1; the interaction is inhibited by CDK2-dependent phosphorylation on Thr-187. Interacts with COPS5, subunit of the COP9 signalosome complex; the interaction leads to CDKN1B degrad
Subcellular Location
Nucleus
Preparation and Storage
-20 degree C for long term storage
Western Blot
Western Blot
Related Product Information for anti-p27 KIP1 antibody
Affinity Purified Phosphorylated Cyclin-dependent kinase inhibitor p27kip1 Antibody C-epitope
Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
Product Categories/Family for anti-p27 KIP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
22,099 Da
NCBI Official Full Name
Cyclin-dependent kinase inhibitor 1B (p27, Kip1)
NCBI Official Synonym Full Names
cyclin-dependent kinase inhibitor 1B
NCBI Official Symbol
CDKN1B
NCBI Official Synonym Symbols
KIP1; MEN4; CDKN4; MEN1B; P27KIP1
NCBI Protein Information
cyclin-dependent kinase inhibitor 1B
UniProt Protein Name
Cyclin-dependent kinase inhibitor p27kip1
UniProt Entry Name
Q9NYG6_HUMAN
NCBI Description
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]
Research Articles on p27 KIP1
Similar Products
Product Notes
The p27 KIP1 (Catalog #AAA540098) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho p27 KIP1 Antibody Affinity Purified reacts with Chicken, Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-p27 KIP1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), IMM, Western Blot (WB). Dot Blot: 1:10,000 ELISA: 1:10,000 Immunoprecipitation: 1:150 Western Blot: 1:250. Researchers should empirically determine the suitability of the p27 KIP1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-p27 KIP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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