Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using ERCC6L Polyclonal Antibody at dilution 1:30)
Rabbit ERCC6L Polyclonal Antibody | anti-ERCC6L antibody
ERCC6L Polyclonal Antibody
Gene Names
ERCC6L; PICH; RAD26L
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry
Purity
Antigen affinity purification
Synonyms
ERCC6L; Polyclonal Antibody; ERCC6L Polyclonal Antibody; PICH; RAD26L; anti-ERCC6L antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen affinity purification
Concentration
1.2mg/mL (varies by lot)
Sequence Length
453
Applicable Applications for anti-ERCC6L antibody
ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
IHC: 1:50-1:200
Immunogen
Recombinant protein of human ERCC6L
Buffer
PBS with 0.05% sodium azide, 50% glycerol, pH7.3
Preparation and Storage
Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using ERCC6L Polyclonal Antibody at dilution 1:30)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using ERCC6L Polyclonal Antibody at dilution 1:30)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human colon cancer tissue using ERCC6L Polyclonal Antibody at dilution 1:30)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human colon cancer tissue using ERCC6L Polyclonal Antibody at dilution 1:30)
Related Product Information for anti-ERCC6L antibody
PICH (Plk1-interacting checkpoint helicase), also known as DNA excision repair protein ERCC-6-like (ERCC6L) or tumor antigen BJ-HCC-15, is a 1, 250 amino acid protein belonging to the SNF2/RAD54 helicase family. PICH is a DNA helicase and an essential component of the spindle assembly checkpoint. During mitosis, PICH recruits MAD2 to kinetochores and also regulates the tension on centromic chromatin. PICH is concentrated in between the kinetochores in prometophase cells, while in metaphase it localizes to the thin threads composed of catenated centromeric DNA that stretch between sister kinetochores. PICH is phosphorylated by Plk, which prevents PICH from associating with chromosome arms and restricts the localization of PICH to the kinetochore-centromere region. PICH/Plk interaction is also required for correct Plk localization to the kinetochore. PICH contains one helicase ATP-binding domain, two TPR repeats and one helicase C-terminal domain.
Product Categories/Family for anti-ERCC6L antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
141,103 Da
NCBI Official Full Name
ERCC6L protein, partial
NCBI Official Synonym Full Names
excision repair cross-complementation group 6-like
NCBI Official Symbol
ERCC6L
NCBI Official Synonym Symbols
PICH; RAD26L
NCBI Protein Information
DNA excision repair protein ERCC-6-like; ATP-dependent helicase ERCC6-like; Plk1-interacting checkpoint helicase; SNF2/RAD54 family protein; excision repair cross-complementing rodent repair deficiency complementation group 6 - like; excision repair cross-complementing rodent repair deficiency, complementation group 6-like; excision repair protein ERCC6-like; tumor antigen BJ-HCC-15
UniProt Protein Name
DNA excision repair protein ERCC-6-like
Protein Family
UniProt Gene Name
ERCC6L
UniProt Synonym Gene Names
PICH
UniProt Entry Name
ERC6L_HUMAN
Uniprot Description
PICH: DNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase. Interacts with PLK1, which phosphorylates it. Both proteins are mutually dependent on each other for correct subcellular localization. Belongs to the SNF2/RAD54 helicase family.
Protein type: EC 3.6.4.12; Helicase
Chromosomal Location of Human Ortholog: Xq13.1
Cellular Component: nucleoplasm; centrosome; membrane; cytoplasm; plasma membrane; cytosol
Molecular Function: protein binding; DNA binding; helicase activity; ATP binding
Biological Process: mitosis; cell division; metabolic process; mitotic cell cycle
Research Articles on ERCC6L
Similar Products
Product Notes
The ERCC6L ercc6l (Catalog #AAA2526417) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ERCC6L Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ERCC6L can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC). IHC: 1:50-1:200. Researchers should empirically determine the suitability of the ERCC6L ercc6l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERCC6L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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