Western Blot (WB)
(Western blot analysis of lysates from A431 cell line, untreated or treated with EGF, 100ng/ml, using Phospho-mouse ERBB2-Y1140 (upper) or Beta-actin (lower).)
Rabbit anti-Human, mouse Phospho-M ERBB2 (Y1140) Polyclonal Antibody | anti-Erbb2 antibody
Phospho-M ERBB2 (Y1140) Antibody
Western Blot (WB)
(Western blot analysis of lysates from A431 cell line, untreated or treated with EGF, 100ng/ml, using Phospho-mouse ERBB2-Y1140 (upper) or Beta-actin (lower).)
Western Blot (WB)
(Western blot analysis of lysates from A431 cell line, untreated or treated with EGF, 100ng/ml, using Phospho-mouse ERBB2-Y1140 (upper) or Beta-actin (lower).)
Western Blot (WB)
(Western blot analysis of extracts from SK-BR-3 cell,untreated or treated with NRG,using phospho-mouse ERBB2-Y1140(left) or mouse ERBB2 antibody(right).)
Western Blot (WB)
(Western blot analysis of extracts from SK-BR-3 cell,untreated or treated with NRG,using phospho-mouse ERBB2-Y1140(left) or mouse ERBB2 antibody(right).)
Dot Blot (DB)
(Dot blot analysis of Phospho-mouse ERBB2-Y1140 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
Dot Blot (DB)
(Dot blot analysis of Phospho-mouse ERBB2-Y1140 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
(EGF) receptor family of receptor tyrosine kinases. This protein
has no ligand binding domain of its own and therefore cannot bind
growth factors. However, it does bind tightly to other ligand-bound
EGF receptor family members to form a heterodimer, stabilizing
ligand binding and enhancing kinase-mediated activation of
downstream signalling pathways, such as those involving
mitogen-activated protein kinase and phosphatidylinositol-3 kinase.
Allelic variations at amino acid positions 654 and 655 of isoform a
(positions 624 and 625 of isoform b) have been reported, with the
most common allele, Ile654/Ile655, shown here. Amplification and/or
overexpression of this gene has been reported in numerous cancers,
including breast and ovarian tumors. Alternative splicing results
in several additional transcript variants, some encoding different
isoforms and others that have not been fully characterized.
Johnson, E., et al. J. Biol. Chem. 285(38):29491-29501(2010)
Huck, L., et al. Proc. Natl. Acad. Sci. U.S.A. 107(35):15559-15564(2010)
Chuang, T.D., et al. J. Biol. Chem. 285(31):23598-23606(2010)
Simeone, L., et al. J. Neurosci. 30(19):6620-6634(2010)
NCBI and Uniprot Product Information
Uniprot Description
HER2: a proto-oncogenic receptor tyrosine kinase of the EGFR family. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. Not activated by EGF, TGF- alpha and amphiregulin. Amplified in breast cancer. Overexpression induces constitutive activity, and the gene is amplified or overexpressed in up to 30% of breast cancers, correlating with poor survival. The antibody Herceptin is approved for treatment of metastatic breast cancer with HER2 amplification/overexpression. Somatic mutations seen in 4% of lung cancers and also in breast, gastric, ovarian cancer and glioblastoma. One SNP shows predisposition to breast and gastric cancer. Inhibitors: Herceptin, lapatinib, PKI-166, EKB-569, CI-1033.
Protein type: Kinase, protein; Protein kinase, TK; EC 2.7.10.1; Oncoprotein; Protein kinase, tyrosine (receptor); Membrane protein, integral; TK group; EGFR family
Cellular Component: microvillus; lateral loop; basolateral plasma membrane; integral to membrane; lipid raft; postsynaptic membrane; membrane; perinuclear region of cytoplasm; cytoplasm; apical plasma membrane; basal plasma membrane; plasma membrane; endosome membrane; cytoplasmic vesicle; nucleus; receptor complex
Molecular Function: protein C-terminus binding; identical protein binding; nucleotide binding; Hsp90 protein binding; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; protein phosphatase binding; protein kinase activity; transferase activity; protein binding; transmembrane receptor activity; protein heterodimerization activity; ubiquitin protein ligase binding; growth factor binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; kinase activity; glycoprotein binding; ATP binding
Biological Process: myelination; phosphoinositide 3-kinase cascade; positive regulation of cell adhesion; peptidyl-tyrosine phosphorylation; positive regulation of translation; wound healing; protein amino acid autophosphorylation; heart development; motor axon guidance; signal transduction; protein amino acid phosphorylation; positive regulation of transcription from RNA polymerase I promoter; positive regulation of MAP kinase activity; sympathetic nervous system development; cell surface receptor linked signal transduction; glial cell differentiation; regulation of transcription, DNA-dependent; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; oligodendrocyte differentiation; response to axon injury; neuromuscular junction development; negative regulation of immature T cell proliferation in the thymus; regulation of microtubule-based process; nervous system development; transcription, DNA-dependent; positive regulation of cell growth; peripheral nervous system development; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of transcription from RNA polymerase III promoter; positive regulation of protein amino acid phosphorylation; positive regulation of Ras protein signal transduction; phosphorylation; positive regulation of epithelial cell proliferation; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis; positive regulation of GTPase activity
Research Articles on Erbb2
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Product Notes
The Erbb2 erbb2 (Catalog #AAA9210880) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-M ERBB2 (Y1140) Antibody reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-M ERBB2 (Y1140) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Dot Blot (DB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the Erbb2 erbb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-M ERBB2 (Y1140), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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