Goat Phospho-I-kappa-B-alpha Polyclonal Antibody | anti-NFKBIA antibody

Phospho-I-kappa-B-alpha Antibody

Cat. Num. AAA9435185

• Gene Names: NFKBIA; IKBA; MAD-3; NFKBI
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunofluorescence
• Purity: Immunogen affinity purified
• Synonyms: Phospho-I-kappa-B-alpha; Polyclonal Antibody; Phospho-I-kappa-B-alpha Antibody; I kappa B alpha antibody; I-kappa-B-alpha antibody; IkappaBalpha antibody; IkB-alpha antibody; IKBA antibody; IKBA_HUMAN antibody; IKBalpha antibody; MAD 3 antibody; MAD3 antibody; Major histocompatibility complex enhancer-binding protein MAD3 antibody; NF kappa B inhibitor alpha antibody; NF-kappa-B inhibitor alpha antibody; NFKBI antibody; NFKBIA antibody; Nuclear factor of kappa light chain gene enhancer in B cells antibody; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody; anti-NFKBIA antibody

• Host: Goat
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen affinity purified
• Form/Format: 1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-NFKBIA antibody: Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
• Application Notes: WB: 1:100-1:1000; IP: 1-2ug per 100-500ug of total protein (1 ml of cell lysate)
• Immunogen: A short amino acid sequence containing Ser 32 phosphorylated Ikappa-B-alpha of human origin.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of phosphorylated-Ikappa-B-alpha expression in Jurkat (A) and TNFalpha/ALLN-treated Jurkat (B) whole cell lysates.)

Related Product Information for anti-NFKBIA antibody

On the basis of both functional and structural considerations, members of the IkappaB family of proteins can be divided into four groups. The first of these groups, IkappaB-alpha, includes the avian protein pp40 and the mammalian MAD-3, both of which inhibit binding of p50-p65 NFkappaB complex or Rel protein to their cognate binding sites but do not inhibit the binding of p50 homodimer to kappaB sites, suggesting that the IkappaB-alpha family binds to the p65 subunit of p50-p65 heterocomplex through Ankyrin repeats. The second member of the IkappaB family is represented by a protein designated IkappaB-beta . The third group of IkappaB proteins is represented by IkappaB-gamma, a protein identical in sequence with the C-terminal domain of the p110 precursor of NFkappaB p50 and expressed predominantly in lymphoid cells. An additional IkappaB family member has been identified as IkappaB-epsilon, a protein which has several phosphorylated forms and is primarily found complexed with RelA and/or c-Rel.

Product Categories/Family for anti-NFKBIA antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 10092619
• NCBI GeneID: 4792
• NCBI Accession #: NP_065390.1
• NCBI GenBank Nucleotide #: NM_020529.2
• UniProt Accession #: P25963; B2R8L6
• Molecular Weight: 35,609 Da
• NCBI Official Full Name: NF-kappa-B inhibitor alpha
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• NCBI Official Symbol: NFKBIA
• NCBI Official Synonym Symbols: IKBA; MAD-3; NFKBI
• NCBI Protein Information: NF-kappa-B inhibitor alpha; ikB-alpha; IkappaBalpha; I-kappa-B-alpha; nuclear factor of kappa light chain gene enhancer in B-cells; major histocompatibility complex enhancer-binding protein MAD3
• UniProt Protein Name: NF-kappa-B inhibitor alpha
• Protein Family: NF-kappa-B inhibitor
• UniProt Gene Name: NFKBIA
• UniProt Synonym Gene Names: IKBA; MAD3; NFKBI; IkB-alpha
• UniProt Entry Name: IKBA_HUMAN

NCBI Description

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]

Uniprot Description

IkB-alpha: a regulatory protein that inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. May be involved in regulation of transcriptional responses to NF-kappa-B, including cell adhesion, immune and proinflammatory responses, apoptosis, differentiation and growth. Controlled by sequential serine-phosphorylation, ubiquitination and degradation.

Protein type: Inhibitor; DNA-binding

Chromosomal Location of Human Ortholog: 14q13

Cellular Component: cytoplasm; plasma membrane; nucleus; cytosol

Molecular Function: identical protein binding; NF-kappaB binding; protein binding; enzyme binding; ubiquitin protein ligase binding; heat shock protein binding; nuclear localization sequence binding; protein complex binding; transcription factor binding

Biological Process: nerve growth factor receptor signaling pathway; viral reproduction; apoptosis; positive regulation of cellular protein metabolic process; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; cytoplasmic sequestering of NF-kappaB; response to exogenous dsRNA; lipopolysaccharide-mediated signaling pathway; positive regulation of interferon type I production; toll-like receptor 4 signaling pathway; cytoplasmic sequestering of transcription factor; negative regulation of myeloid cell differentiation; MyD88-independent toll-like receptor signaling pathway; negative regulation of DNA binding; regulation of NF-kappaB import into nucleus; toll-like receptor 2 signaling pathway; regulation of cell proliferation; MyD88-dependent toll-like receptor signaling pathway; response to muramyl dipeptide; protein import into nucleus, translocation; inhibition of NF-kappaB transcription factor; toll-like receptor signaling pathway; negative regulation of Notch signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; negative regulation of apoptosis

Disease: Ectodermal Dysplasia, Anhidrotic, With T-cell Immunodeficiency, Autosomal Dominant