Rabbit anti-Human IKBalpha Polyclonal Antibody | anti-IKBalpha antibody

IKBalpha (Phospho-S32) Polyclonal Antibody

Cat. Num. AAA3013938

• Gene Names: NFKBIA; IKBA; MAD-3; NFKBI; EDAID2
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunoprecipitation
• Purity: ProA affinity purified
• Synonyms: IKBalpha; Polyclonal Antibody; IKBalpha (Phospho-S32) Polyclonal Antibody; IkB-alpha; IkappaBalpha; NFKBIA; IKBA; MAD3; NFKBI; I kappa B alpha; I-kappa-B-alpha; MAD 3; Major histocompatibility complex enhancer-binding protein MAD3; NF kappa B inhibitor alpha; NF-kappa-B inhibitor alpha; Nuclear factor of kappa light chain gene enhancer in B cells; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha; anti-IKBalpha antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: IKBalpha (Phospho-S32) polyclonal antibody detects endogenous levels of IKBalpha protein only when phosphorylated at S32.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 317

• Applicable Applications for anti-IKBalpha antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Application Notes: WB: 1:1,000; ICC: 1:50-1:200
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser32 of human IKB alpha.
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of Phospho-IKB alpha (S32) on Ags cell lysates using anti-Phospho-IKB alpha (S32) antibody at 1/1,000 dilution.)

Immunocytochemistry (ICC)

(ICC staining Phospho-IKB alpha (S32) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-IKBalpha antibody

On the basis of both functional and structural considerations, members of the IkappaB family of proteins can be divided into four groups. The first of these groups, IkappaB-alpha, includes the avian protein pp40 and the mammalian MAD-3, both of which inhibit binding of p50-p65 NFkB complex or Rel protein to their cognate binding sites but do not inhibit the binding of p50 homodimer to kappaB sites, suggesting that the IkappaB-alpha family binds to the p65 subunit of p50-p65 heterocomplex through ankyrin repeats. The second member of the IkappaB family is represented by a protein designated IkappaB-beta. The third group of IkappaB proteins is represented by IkappaB-gamma, which is identical in sequence with the C-terminal domain of the p110 precursor of NFkB p50 and is expressed predominantly in lymphoid cells. An additional IkappaB family member, IkappaB-epsilon, has several phosphorylated forms and is primarily found complexed with Rel A and/or c-Rel.

NCBI and Uniprot Product Information

• NCBI GI #: 10092619
• NCBI GeneID: 4792
• NCBI Accession #: NP_065390.1
• NCBI GenBank Nucleotide #: NP_065390.1
• UniProt Accession #: P25963
• Molecular Weight: 36kDa
• NCBI Official Full Name: NF-kappa-B inhibitor alpha
• NCBI Official Synonym Full Names: NFKB inhibitor alpha
• NCBI Official Symbol: NFKBIA
• NCBI Official Synonym Symbols: IKBA; MAD-3; NFKBI; EDAID2
• NCBI Protein Information: NF-kappa-B inhibitor alpha
• UniProt Protein Name: NF-kappa-B inhibitor alpha
• UniProt Gene Name: NFKBIA
• UniProt Synonym Gene Names: IKBA; MAD3; NFKBI; IkB-alpha
• UniProt Entry Name: IKBA_HUMAN

NCBI Description

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]

Uniprot Description

IkB-alpha: a regulatory protein that inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. May be involved in regulation of transcriptional responses to NF-kappa-B, including cell adhesion, immune and proinflammatory responses, apoptosis, differentiation and growth. Controlled by sequential serine-phosphorylation, ubiquitination and degradation.

Protein type: Inhibitor; DNA-binding

Chromosomal Location of Human Ortholog: 14q13

Cellular Component: cytoplasm; plasma membrane; nucleus; cytosol

Molecular Function: identical protein binding; NF-kappaB binding; protein binding; enzyme binding; ubiquitin protein ligase binding; heat shock protein binding; nuclear localization sequence binding; protein complex binding; transcription factor binding

Biological Process: nerve growth factor receptor signaling pathway; viral reproduction; apoptosis; positive regulation of cellular protein metabolic process; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; cytoplasmic sequestering of NF-kappaB; response to exogenous dsRNA; lipopolysaccharide-mediated signaling pathway; positive regulation of interferon type I production; toll-like receptor 4 signaling pathway; cytoplasmic sequestering of transcription factor; negative regulation of myeloid cell differentiation; MyD88-independent toll-like receptor signaling pathway; negative regulation of DNA binding; regulation of NF-kappaB import into nucleus; toll-like receptor 2 signaling pathway; regulation of cell proliferation; MyD88-dependent toll-like receptor signaling pathway; response to muramyl dipeptide; protein import into nucleus, translocation; inhibition of NF-kappaB transcription factor; toll-like receptor signaling pathway; negative regulation of Notch signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; negative regulation of apoptosis

Disease: Ectodermal Dysplasia, Anhidrotic, With T-cell Immunodeficiency, Autosomal Dominant