Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using Phospho-GYS1-S641 antibody at 1:1000 dilution.NIH/3T3 cells and C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
Rabbit Phospho-GYS1-S641 Polyclonal Antibody | anti-GYS1-S641 antibody
Phospho-GYS1-S641 Rabbit pAb
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using Phospho-GYS1-S641 antibody at 1:1000 dilution.NIH/3T3 cells and C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using Phospho-GYS1-S641 antibody at 1:1000 dilution.NIH/3T3 cells and C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
Western Blot (WB)
(Western blot analysis of extracts of 293T cells, using Phospho-GYS1-S641 pAb at 1:1000 dilution or Glycogen synthase 1 (GYS1) antibody (A2519).293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
Western Blot (WB)
(Western blot analysis of extracts of 293T cells, using Phospho-GYS1-S641 pAb at 1:1000 dilution or Glycogen synthase 1 (GYS1) antibody (A2519).293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene catalyzes the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages. Mutations in this gene are associated with muscle glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
Uniprot Description
GYS1: muscle glycogen synthase 1. Transfers glucosyl residue from UDP-glucose to glycogen. Regulated allosterically by glucose-6-phosphate, and by PKA-mediated phosphorylation.
Protein type: EC 2.4.1.11; Transferase; Carbohydrate Metabolism - starch and sucrose
Chromosomal Location of Human Ortholog: 19q13.3
Cellular Component: membrane; cytoplasm; inclusion body; cytosol
Molecular Function: protein binding; glycogen (starch) synthase activity; protein kinase binding; glucose binding
Biological Process: glycogen biosynthetic process; heart development; carbohydrate metabolic process; glucose metabolic process; pathogenesis
Disease: Glycogen Storage Disease 0, Muscle
Research Articles on GYS1-S641
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Product Notes
The GYS1-S641 gys1 (Catalog #AAA9143781) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-GYS1-S641 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-GYS1-S641 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the GYS1-S641 gys1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-GYS1-S641, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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