Dot Blot (DB)
(Dot blot analysis of Phospho-BAD-S74 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
Rabbit anti-Human Phospho-BAD (S74) Polyclonal Antibody | anti-BAD antibody
Phospho-BAD (S74) Antibody
Dot Blot (DB)
(Dot blot analysis of Phospho-BAD-S74 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
Dot Blot (DB)
(Dot blot analysis of Phospho-BAD-S74 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
family. BCL-2 family members are known to be regulators of
programmed cell death. This protein positively regulates cell
apoptosis by forming heterodimers with BCL-xL and BCL-2, and
reversing their death repressor activity. Proapoptotic activity of
this protein is regulated through its phosphorylation. Protein
kinases AKT and MAP kinase, as well as protein phosphatase
calcineurin were found to be involved in the regulation of this
protein. Alternative splicing of this gene results in two
transcript variants which encode the same isoform. [provided by
RefSeq].
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Galmiche, A., et al. Mol. Cancer Res. 8(8):1116-1125(2010)
Cerioni, L., et al. Methods Mol. Biol. 648, 291-301 (2010) :
Yu, B., et al. J. Exp. Clin. Cancer Res. 29, 107 (2010) :
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq, Jul 2008]
Uniprot Description
BAD: a proapoptotic member of the Bcl-2 family. Displaces Bax from binding to Bcl-2 and Bcl-xL, resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad inducing the phosphorylation of Bad by Akt and p90RSK. 14-3-3 proteins bind phosphorylated Bad, inhibiting its binding to Bcl-2 and Bcl-xL. Phosphorylation by mitochondria-anchored PKA in the BH3 domain can block the dimerization of Bad and Bcl-xL.
Protein type: Apoptosis
Chromosomal Location of Human Ortholog: 11q13.1
Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol
Molecular Function: protein kinase B binding; protein binding; protein heterodimerization activity; phospholipid binding; caspase activator activity; lipid binding; protein kinase binding; protein phosphatase 2B binding
Biological Process: response to oleate; nerve growth factor receptor signaling pathway; apoptosis; positive regulation of proteolysis; positive regulation of apoptosis; response to glucocorticoid stimulus; positive regulation of caspase activity; glucose homeostasis; cellular process regulating host cell cycle in response to virus; response to estradiol stimulus; positive regulation of apoptosis by virus; response to glucose stimulus; pore complex biogenesis; positive regulation of autophagy; positive regulation of glucokinase activity; caspase activation; epidermal growth factor receptor signaling pathway; response to drug; release of cytochrome c from mitochondria; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; cytokine and chemokine mediated signaling pathway; suppression by virus of host apoptosis; ADP metabolic process; positive regulation of insulin secretion; response to testosterone stimulus; response to amino acid stimulus; ATP metabolic process; glucose catabolic process; response to ethanol; induction of apoptosis via death domain receptors; response to hydrogen peroxide; DNA damage response, signal transduction resulting in induction of apoptosis; positive regulation of T cell differentiation; innate immune response; positive regulation of B cell differentiation; regulation of mitochondrial membrane permeability; response to calcium ion; response to progesterone stimulus; positive regulation of epithelial cell proliferation
Research Articles on BAD
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Product Notes
The BAD bad (Catalog #AAA9209262) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-BAD (S74) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-BAD (S74) can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), ELISA (EIA). DB~~1:500. Researchers should empirically determine the suitability of the BAD bad for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-BAD (S74), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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