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Western Blot (WB) (Western blotAll lanes: Phosphatidylinositol-glycan biosynthesis class X protein antibody at 2ug/mlLane 1:mouse kidney tissueLane 2:293T whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 29,31 kDaObserved band size: 29 kDa)

Rabbit anti-Human Phosphatidylinositol-glycan biosynthesis class X protein Polyclonal Antibody | anti-PIGX antibody

Rabbit anti-human Phosphatidylinositol-glycan biosynthesis class X protein polyclonal Antibody

Gene Names
PIGX; PIG-X
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Synonyms
Phosphatidylinositol-glycan biosynthesis class X protein; Polyclonal Antibody; Rabbit anti-human Phosphatidylinositol-glycan biosynthesis class X protein polyclonal Antibody; PIG-X; PIGX; anti-PIGX antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Liquid
Sequence Length
276
Applicable Applications for anti-PIGX antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Recommended dilution: WB:1:500-2000, IHC:1:20-1:200
Immunogen
Recombinant human Phosphatidylinositol-glycan biosynthesis class X protein(42-230aa)
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-100097 / sc-100096
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western blotAll lanes: Phosphatidylinositol-glycan biosynthesis class X protein antibody at 2ug/mlLane 1:mouse kidney tissueLane 2:293T whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 29,31 kDaObserved band size: 29 kDa)

Western Blot (WB) (Western blotAll lanes: Phosphatidylinositol-glycan biosynthesis class X protein antibody at 2ug/mlLane 1:mouse kidney tissueLane 2:293T whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 29,31 kDaObserved band size: 29 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human placenta tissue using MBS7002365 at dilution 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human placenta tissue using MBS7002365 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human rectum tissue using MBS7002365 at dilution 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human rectum tissue using MBS7002365 at dilution 1:100)
Related Product Information for anti-PIGX antibody
Essential component of glycosylphosphatidylinositol-mannosyltransferase 1 which transfers the first of the 4 mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Probably acts by stabilizing the mannosyltransferase PIGM.

This gene encodes a type I transmembrane protein in the endoplasmic reticulum (ER). The protein is an essential component of glycosylphosphatidylinositol-mannosyltransferase I, which transfers the first of the four mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Studies in rat indicate that the protein is translated from a non-AUG translation initiation site. Alternative splicing results in multiple transcript variants.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30,974 Da
NCBI Official Full Name
phosphatidylinositol-glycan biosynthesis class X protein isoform 1
NCBI Official Synonym Full Names
phosphatidylinositol glycan anchor biosynthesis class X
NCBI Official Symbol
PIGX
NCBI Official Synonym Symbols
PIG-X
NCBI Protein Information
phosphatidylinositol-glycan biosynthesis class X protein
UniProt Protein Name
Phosphatidylinositol-glycan biosynthesis class X protein
UniProt Gene Name
PIGX
UniProt Synonym Gene Names
PIG-X
UniProt Entry Name
PIGX_HUMAN

NCBI Description

This gene encodes a type I transmembrane protein in the endoplasmic reticulum (ER). The protein is an essential component of glycosylphosphatidylinositol-mannosyltransferase I, which transfers the first of the four mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Studies in rat indicate that the protein is translated from a non-AUG translation initiation site. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Uniprot Description

PIGX: Essential component of glycosylphosphatidylinositol- mannosyltransferase 1 which transfers the first of the 4 mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Probably acts by stabilizing the mannosyltransferase PIGM. Belongs to the PIGX family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Biological Process: C-terminal protein lipidation; cellular protein metabolic process; post-translational protein modification; preassembly of GPI anchor in ER membrane

Research Articles on PIGX

Similar Products

Product Notes

The PIGX pigx (Catalog #AAA7002365) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Phosphatidylinositol-glycan biosynthesis class X protein polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Phosphatidylinositol-glycan biosynthesis class X protein can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Recommended dilution: WB:1:500-2000, IHC:1:20-1:200. Researchers should empirically determine the suitability of the PIGX pigx for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phosphatidylinositol-glycan biosynthesis class X protein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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