Rabbit Phosphatase, Alkaline, Tissue-nonspecific Isozyme Polyclonal Antibody | anti-ALPL antibody

Phosphatase, Alkaline, Tissue-nonspecific Isozyme (AP-TNAP, ALPL, TNSALP, Alkaline Phosphatase Liver/Bone/Kidney Isozyme)

Cat. Num. AAA626926

• Gene Names: ALPL; ALP
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Phosphatase; Alkaline; Tissue-nonspecific Isozyme; Polyclonal Antibody; Tissue-nonspecific Isozyme (AP-TNAP; ALPL; TNSALP; Alkaline Phosphatase Liver/Bone/Kidney Isozyme); Anti -Phosphatase; anti-ALPL antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ALPL. Species Cross-Reactivity: mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with sterile dH2O.

• Applicable Applications for anti-ALPL antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot:1-2ug/ml; Immunohistochemistry (paraffin):1-2ug/ml
• Immunogen: Synthetic peptide corresponding to a sequence at the N-terminal of human ALPL, different from the related rat and mouse sequence by two aa.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile dH20. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Rat Tissue sections (samll intestine) Staining ALPL in cytoplasm DAB chromogenic reaction)

Western Blot (WB)

(Western blot analysis of Hela cell Ttissue lysis Using ALPL antibody)

Related Product Information for anti-ALPL antibody

Alkaline phosphatase (ALPL) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts,the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine (PEA) and pyridoxal-5-prime-phosphate (PLP). ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb.Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.

Product Categories/Family for anti-ALPL antibody

Antibodies; Abs to Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 545490188
• NCBI GeneID: 403548
• NCBI Accession #: XP_005617271.1
• NCBI GenBank Nucleotide #: XM_005617214.1
• NCBI Official Full Name: alkaline phosphatase, tissue-nonspecific isozyme isoform X3
• NCBI Official Symbol: ALPL
• NCBI Official Synonym Symbols: ALP
• NCBI Protein Information: alkaline phosphatase, tissue-nonspecific isozyme; tissue non-specific alkaline phosphatase
• Protein Family: Alkaline phosphatase