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Western Blot (WB) (Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-3: Hela cells, 293T cells, HepG2 cells, Primary antibody: 862047(PHKG1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)

Rabbit anti-Human PHKG1 Polyclonal Antibody | anti-PHKG1 antibody

PHKG1 Rabbit pAb

Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity purified
Synonyms
PHKG1; Polyclonal Antibody; PHKG1 Rabbit pAb; anti-PHKG1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
-20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-PHKG1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB: 1:200-1:1000
Immunogen
Synthetic peptide of human PHKG1
Conjugation
Unconjugated
Molecular Function
This gene is a member of the Ser/Thr protein kinase family and encodes a protein with one protein kinase domain and two calmodulin-binding domains. This protein is the catalytic member of a 16 subunit protein kinase complex which contains equimolar ratios of 4 subunit types. The complex is a crucial glycogenolytic regulatory enzyme. This gene has two pseudogenes at chromosome 7q11.21 and one at chromosome 11p11.12. Multiple transcript variants encoding different isoforms have been found for this gene.
Modification
Unmodified
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-3: Hela cells, 293T cells, HepG2 cells, Primary antibody: 862047(PHKG1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)

Western Blot (WB) (Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-3: Hela cells, 293T cells, HepG2 cells, Primary antibody: 862047(PHKG1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)
Product Categories/Family for anti-PHKG1 antibody

NCBI and Uniprot Product Information

UniProt Accession #
Molecular Weight
45kDa
UniProt Protein Name
Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform
Protein Family
UniProt Gene Name
PHKG1
UniProt Synonym Gene Names
PHKG; PHK-gamma-M
UniProt Entry Name
PHKG1_HUMAN

Uniprot Description

PHKG1: a Ca(2+)/calmodulin-dependent protein kinase of the PHK family. Phosphorylase kinase is a polymer of 16 chains, four each of alpha, beta, gamma, and delta. Alpha and beta are regulatory chains, gamma is the catalytic chain, and delta is calmodulin.

Protein type: Kinase, protein; Protein kinase, CAMK; EC 2.7.11.26; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; EC 2.7.11.19; CAMK group; PHK family

Chromosomal Location of Human Ortholog: 7p11.2

Cellular Component: phosphorylase kinase complex; cytosol

Molecular Function: calmodulin binding; phosphorylase kinase activity; enzyme binding; tau-protein kinase activity; ATP binding

Biological Process: glycogen biosynthetic process; glycogen catabolic process; carbohydrate metabolic process; glucose metabolic process; pathogenesis; protein amino acid phosphorylation

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Product Notes

The PHKG1 phkg1 (Catalog #AAA4758502) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PHKG1 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PHKG1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 1:200-1:1000. Researchers should empirically determine the suitability of the PHKG1 phkg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHKG1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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