Western Blot (WB)
(Figure 1. Western blot analysis of PHF21B using anti-PHF21B antibody (MBS1753844).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human U20S whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-PHF21B antigen affinity purified polyclonal antibody (Catalog # MBS1753844) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for PHF21B at approximately 57KD. The expected band size for PHF21B is at 57KD. )
Rabbit anti-Human PHF21B Polyclonal Antibody | anti-PHF21B antibody
Anti-PHF21B Antibody
Gene Names
PHF21B; PHF4; BHC80L
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, ELISA
Purity
Immunogen affinity purified.
Synonyms
PHF21B; Polyclonal Antibody; Anti-PHF21B Antibody; KIAA1661; PHD finger protein 21B; anti-PHF21B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rabbit IgG polyclonal antibody for PHF21B detection.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Applicable Applications for anti-PHF21B antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Direct ELISA (EIA)
Application Notes
WB: 0.25-0.5ug/ml|Human|
ICC/IF: 5ug/ml|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|
ICC/IF: 5ug/ml|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|
Immunogen
E Coli-derived human PHF21B recombinant protein (Position: E291-M499).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems
Recommended Detection Systems
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Figure 1. Western blot analysis of PHF21B using anti-PHF21B antibody (MBS1753844).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human U20S whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-PHF21B antigen affinity purified polyclonal antibody (Catalog # MBS1753844) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for PHF21B at approximately 57KD. The expected band size for PHF21B is at 57KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of PHF21B using anti-PHF21B antibody (MBS1753844).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human U20S whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-PHF21B antigen affinity purified polyclonal antibody (Catalog # MBS1753844) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for PHF21B at approximately 57KD. The expected band size for PHF21B is at 57KD. )
Immunofluorescence (IF)
(Figure 2. IF analysis of PHF21B using anti- PHF21B antibody (MBS1753844).PHF21B was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- PHF21B Antibody (MBS1753844) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. )
Immunofluorescence (IF)
(Figure 2. IF analysis of PHF21B using anti- PHF21B antibody (MBS1753844).PHF21B was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- PHF21B Antibody (MBS1753844) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. )
Related Product Information for anti-PHF21B antibody
PHF21B (PHD finger protein 21B), also known as PHF4, is a 531 amino acid protein that contains one PHD-type zinc finger and is expressed as multiple alternatively spliced isoforms. The gene encoding PHF21B maps to human chromosome 22, which houses over 500 genes and is the second smallest human chromosome. Mutations in several of the genes that map to chromosome 22 are involved in the development of Phelan-McDermid syndrome, neurofibromatosis type 2, autism and schizophrenia.
References
1. Bergamo, N. A., da Silva Veiga, L. C., dos Reis, P. P., Nishimoto, I. N., Magrin, J., Kowalski, L. P., Squire, J. A., Rogatto, S. R. Classic and molecular cytogenetic analyses reveal chromosomal gains and losses correlated with survival in head and neck cancer patients. Clin. Cancer Res. 11: 621-631, 2005.
2. Bertonha, F. B., Barros Filho, M. deC., Kuasne, H., dos Reis, P. P., da Costa Prando, E., Munoz, J. J. A. M., Roffe, M., Hajj, G. N. M., Kowalski, L. P., Rainho, C. A., Rogatto, S. R. PHF21B as a candidate tumor suppressor gene in head and neck squamous cell carcinomas. Molec. Oncol. 9: 450-462, 2015.
3. Reis, P. P., Rogatto, S. R., Kowalski, L. P., Nishimoto, I. N., Montovani, J. C., Corpus, G., Squire, J. A., Kamel-Reid, S. Quantitative real-time PCR identifies a critical region of deletion on 22q13 related to prognosis in oral cancer. Oncogene 21: 6480-6487, 2002.
2. Bertonha, F. B., Barros Filho, M. deC., Kuasne, H., dos Reis, P. P., da Costa Prando, E., Munoz, J. J. A. M., Roffe, M., Hajj, G. N. M., Kowalski, L. P., Rainho, C. A., Rogatto, S. R. PHF21B as a candidate tumor suppressor gene in head and neck squamous cell carcinomas. Molec. Oncol. 9: 450-462, 2015.
3. Reis, P. P., Rogatto, S. R., Kowalski, L. P., Nishimoto, I. N., Montovani, J. C., Corpus, G., Squire, J. A., Kamel-Reid, S. Quantitative real-time PCR identifies a critical region of deletion on 22q13 related to prognosis in oral cancer. Oncogene 21: 6480-6487, 2002.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,776 Da
NCBI Official Full Name
PHD finger protein 21B isoform 1
NCBI Official Synonym Full Names
PHD finger protein 21B
NCBI Official Symbol
PHF21B
NCBI Official Synonym Symbols
PHF4; BHC80L
NCBI Protein Information
PHD finger protein 21B
UniProt Protein Name
PHD finger protein 21B
Protein Family
UniProt Gene Name
PHF21B
UniProt Synonym Gene Names
KIAA1661
UniProt Entry Name
PF21B_HUMAN
Uniprot Description
PHF21B: 3 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 22q13.31
Molecular Function: zinc ion binding
Research Articles on PHF21B
Similar Products
Product Notes
The PHF21B phf21b (Catalog #AAA1753844) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PHF21B Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PHF21B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Direct ELISA (EIA). WB: 0.25-0.5ug/ml|Human| ICC/IF: 5ug/ml|Human| Direct ELISA: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the PHF21B phf21b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHF21B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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