Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PHF21B protein (Right) or empty vector (Left) detected with PHF21B antibody)
Mouse PHF21B Monoclonal Antibody | anti-PHF21B antibody
PHF21B antibody
Gene Names
PHF21B; PHF4; BHC80L
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
PHF21B antibody was purified by affinity chromatography.
Synonyms
PHF21B; Monoclonal Antibody; PHF21B antibody; Monoclonal PHF21B; Anti-PHF21B; PHD finger protein 21B; BHC80L; PHD finger protein 4; PHF4; KIAA1661; PHFB 21; PHFB-21; anti-PHF21B antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
3D1
Purity/Purification
PHF21B antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
531
Applicable Applications for anti-PHF21B antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC: 1:100
IF: 1:100
IHC: 1:150
WB: 1:2000
IF: 1:100
IHC: 1:150
WB: 1:2000
Biological Significance
The function of PHF21B has not been widely studied, and is yet to be fully elucidated.
Cross-Reactivity
Human
Immunogen
PHF21B antibody was raised in mouse using a full length recombinant protein of human PHF21B (NP_612424) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PHF21B protein (Right) or empty vector (Left) detected with PHF21B antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PHF21B protein (Right) or empty vector (Left) detected with PHF21B antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PHF21B protein in paraffin embedded Human Kidney tissue using PHF21B antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PHF21B protein in paraffin embedded Human Kidney tissue using PHF21B antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant PHF21B protein using PHF21B antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant PHF21B protein using PHF21B antibody)
Related Product Information for anti-PHF21B antibody
Mouse monoclonal PHF21B antibody
Product Categories/Family for anti-PHF21B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,776 Da
NCBI Official Full Name
PHD finger protein 21B isoform 1
NCBI Official Synonym Full Names
PHD finger protein 21B
NCBI Official Symbol
PHF21B
NCBI Official Synonym Symbols
PHF4; BHC80L
NCBI Protein Information
PHD finger protein 21B
UniProt Protein Name
PHD finger protein 21B
Protein Family
UniProt Gene Name
PHF21B
UniProt Synonym Gene Names
KIAA1661
UniProt Entry Name
PF21B_HUMAN
Uniprot Description
PHF21B: 3 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 22q13.31
Molecular Function: zinc ion binding
Research Articles on PHF21B
Similar Products
Product Notes
The PHF21B phf21b (Catalog #AAA832250) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PHF21B can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). FC: 1:100 IF: 1:100 IHC: 1:150 WB: 1:2000. Researchers should empirically determine the suitability of the PHF21B phf21b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHF21B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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