Western Blot (WB)
(PHF12 Antibody (C-term) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the PHF12 antibody detected the PHF12 protein (arrow).)
Rabbit anti-Mouse PHF12 Polyclonal Antibody | anti-PHF12 antibody
PHF12 Antibody (C-term)
Gene Names
PHF12; PF1
Reactivity
Mouse
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PHF12; Polyclonal Antibody; PHF12 Antibody (C-term); PHD finger protein 12; PHD factor 1; Pf1; PHF12 HGNC:20816); anti-PHF12 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PHF12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 831-859 amino acids from the C-terminal region of human PHF12.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
831-859
Sequence Length
849
Applicable Applications for anti-PHF12 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(PHF12 Antibody (C-term) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the PHF12 antibody detected the PHF12 protein (arrow).)
Western Blot (WB)
(PHF12 Antibody (C-term) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the PHF12 antibody detected the PHF12 protein (arrow).)
Immunohistochemistry (IHC)
(PHF12 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PHF12 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(PHF12 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PHF12 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-PHF12 antibody
Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
References
Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006)
Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Yochum, G.S., et al. Mol. Cell. Biol. 22(22):7868-7876(2002)
Yochum, G.S., et al. Mol. Cell. Biol. 21(13):4110-4118(2001)
Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Yochum, G.S., et al. Mol. Cell. Biol. 22(22):7868-7876(2002)
Yochum, G.S., et al. Mol. Cell. Biol. 21(13):4110-4118(2001)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
109698
NCBI Official Full Name
PHD finger protein 12 isoform 1
NCBI Official Synonym Full Names
PHD finger protein 12
NCBI Official Symbol
PHF12
NCBI Official Synonym Symbols
PF1
NCBI Protein Information
PHD finger protein 12
UniProt Protein Name
PHD finger protein 12
Protein Family
UniProt Gene Name
PHF12
UniProt Synonym Gene Names
Pf1
UniProt Entry Name
PHF12_HUMAN
Uniprot Description
PHF12: Acts as a transcriptional repressor. Involved in recruitment of functional SIN3A complexes to DNA. Represses transcription at least in part through the activity of an associated histone deacetylase (HDAC). May also repress transcription in a SIN3A-independent manner through recruitment of functional AES complexes to DNA. 4 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 17q11.2
Cellular Component: nucleoplasm; Sin3 complex; transcriptional repressor complex; nucleolus; nucleus
Molecular Function: protein binding; zinc ion binding; phosphoinositide binding
Biological Process: transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent
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Product Notes
The PHF12 phf12 (Catalog #AAA9207541) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 831-859. The PHF12 Antibody (C-term) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PHF12 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the PHF12 phf12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHF12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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