Rabbit anti-Mouse PHF12 Polyclonal Antibody | anti-PHF12 antibody

PHF12, CT (PHF12, KIAA1523, PHD finger protein 12, PHD factor 1) (AP)

Cat. Num. AAA6333266

• Gene Names: PHF12; PF1
• Reactivity: Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PHF12; Polyclonal Antibody; CT (PHF12; KIAA1523; PHD finger protein 12; PHD factor 1) (AP); PHD factor 1; anti-PHF12 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-PHF12 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PHF12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 838-866 amino acids from the C-terminal region of human PHF12.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PHF12 Antibody (C-term) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the PHF12 antibody detected the PHF12 protein (arrow).)

Immunohistochemistry (IHC)

(PHF12 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PHF12 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PHF12 antibody

Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.

Product Categories/Family for anti-PHF12 antibody

Antibodies; KIAA; orf

References

Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006). Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006). Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004). Yochum, G.S., et al. Mol. Cell. Biol. 22(22):7868-7876(2002). Yochum, G.S., et al. Mol. Cell. Biol. 21(13):4110-4118(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 75677357
• NCBI GeneID: 57649
• NCBI Accession #: NP_001028733.1; NP_065940.1
• NCBI GenBank Nucleotide #: NM_001033561.1
• UniProt Accession #: Q96QT6; Q0VAI5; Q6ZML2; Q9BV34; Q9H7U9; Q9P205
• Molecular Weight: 109,698 Da
• NCBI Official Full Name: PHD finger protein 12 isoform 1
• NCBI Official Synonym Full Names: PHD finger protein 12
• NCBI Official Symbol: PHF12
• NCBI Official Synonym Symbols: PF1
• NCBI Protein Information: PHD finger protein 12; PHD factor 1; PHD zinc finger transcription factor
• UniProt Protein Name: PHD finger protein 12
• Protein Family: PHD finger protein
• UniProt Gene Name: PHF12
• UniProt Synonym Gene Names: KIAA1523; Pf1
• UniProt Entry Name: PHF12_HUMAN

Uniprot Description

Function: Acts as a transcriptional repressor. Involved in recruitment of functional SIN3A complexes to DNA. Represses transcription at least in part through the activity of an associated histone deacetylase (HDAC). May also repress transcription in a SIN3A-independent manner through recruitment of functional AES complexes to DNA. Ref.1

Subunit structure: Isoform 2 interacts with SIN3A in a complex composed of HDAC1, SAP30 and SIN3A. Interacts with AES. Ref.1

Subcellular location: Nucleus Ref.1.

Domain: The polybasic region (PBR) is responsive to the binding to phosphoinositides (PtdInsPs).

Sequence similarities: Contains 1 FHA domain.Contains 2 PHD-type zinc fingers.