Rabbit PHF1 Polyclonal Antibody | anti-PHF1 antibody
PHF1 Conjugated Antibody
Gene Names
PHF1; PCL1; PHF2; hPHF1; MTF2L2; TDRD19C
Reactivity
Human, Mouse, Rat
Applications
Most Applications
Synonyms
PHF1; Polyclonal Antibody; PHF1 Conjugated Antibody; PHD finger protein 1; Protein PHF1; Polycomb-like protein 1; hPCl1; anti-PHF1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total PHF1 protein.
Form/Format
0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
Applicable Applications for anti-PHF1 antibody
Most Applications
Application Notes
Suggested Dilution:
AF350 conjugated: most applications: 1:50-1:250
AF405 conjugated: most applications: 1:50-1:250
AF488 conjugated: most applications: 1:50-1:250
AF555 conjugated: most applications: 1:50-1:250
AF594 conjugated: most applications: 1:50-1:250
AF647 conjugated: most applications: 1:50-1:250
AF680 conjugated: most applications: 1:50-1:250
AF750 conjugated: most applications: 1:50-1:250
Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1:50-1:1000
AF350 conjugated: most applications: 1:50-1:250
AF405 conjugated: most applications: 1:50-1:250
AF488 conjugated: most applications: 1:50-1:250
AF555 conjugated: most applications: 1:50-1:250
AF594 conjugated: most applications: 1:50-1:250
AF647 conjugated: most applications: 1:50-1:250
AF680 conjugated: most applications: 1:50-1:250
AF750 conjugated: most applications: 1:50-1:250
Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1:50-1:1000
Immunogen
Synthesized peptide derived from internal of human PHF1.
Conjugation
Biotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
Excitation Emission
AF350: 346nm/442nm
Preparation and Storage
Store at 4 degree C for 6 months.
Related Product Information for anti-PHF1 antibody
Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Involved in DNA damage response and is recruited at double-strand breaks (DSBs). Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex: it is however unclear whether recruitment of the PRC2 complex to H3K36me3 leads to enhance or inhibit H3K27me3 methylation mediated by the PRC2 complex. According to some reports, PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 loci (and). According to another report, PHF1 recruits the PRC2 complex at double-strand breaks (DSBs) and inhibits the activity of PRC2 (). Regulates p53/TP53 stability and prolonges its turnover: may act by specifically binding to a methylated from of p53/TP53.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62,106 Da
NCBI Official Full Name
PHD finger protein 1 isoform a
NCBI Official Synonym Full Names
PHD finger protein 1
NCBI Official Symbol
PHF1
NCBI Official Synonym Symbols
PCL1; PHF2; hPHF1; MTF2L2; TDRD19C
NCBI Protein Information
PHD finger protein 1; hPCl1; polycomb-like protein 1; tudor domain containing 19C
UniProt Protein Name
PHD finger protein 1
Protein Family
UniProt Gene Name
PHF1
UniProt Synonym Gene Names
PCL1; Protein PHF1; hPHF1; hPCl1
UniProt Entry Name
PHF1_HUMAN
NCBI Description
This gene encodes a Polycomb group protein. The protein is a component of a histone H3 lysine-27 (H3K27)-specific methyltransferase complex, and functions in transcriptional repression of homeotic genes. The protein is also recruited to double-strand breaks, and reduced protein levels results in X-ray sensitivity and increased homologous recombination. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
Uniprot Description
PHF1: Transcriptional repressor. May promote methylation of histone H3 on 'Lys-27' by the PRC2/EED-EZH2 complex. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor
Chromosomal Location of Human Ortholog: 6p21.3
Cellular Component: nucleoplasm; ESC/E(Z) complex; cytoplasm; microtubule organizing center; nucleus
Molecular Function: zinc ion binding; transcription factor activity; methylated histone residue binding
Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; negative regulation of gene expression, epigenetic; gene expression; chromatin modification; response to DNA damage stimulus; regulation of gene expression, epigenetic
