SDS-Page
(Recombinant PHD1 / EGLN2 protein gel 10% SDS-PAGE Coomassie staining MW: 44.9 kDa Purity: >90%)
PHD1 recombinant protein
Recombinant PHD1 (EGLN2) protein
Gene Names
EGLN2; EIT6; PHD1; HPH-1; HPH-3; HIFPH1; HIF-PH1
Synonyms
PHD1; Recombinant PHD1 (EGLN2) protein; prolyl-4-hydroxylase 1; Egl-9 Family Hypoxia Inducible Factor 2; HIF-PH1; PHD1 recombinant protein
Host
Baculovirus
Form/Format
Recombinant PHD1/EGLN2 protein is in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
This protein is suitable for use in binding assays, inhibitor screening, and selectivity profiling. Assay Conditions: 3uM HIF1A (HIF-1a) peptide (DLDLEALAPYIPADDDFQL) was incubated with 300nm PHD1 protein in 30ul reaction system containing 20mM Tris-HCl pH 7.5, 5mM KCl, 1.5mM MgCl2, 1mM DTT, 100uM 2-oxoglutarate, 100uM ascorbate and 50uM (NH4)2Fe(SO4)2·6H2O for 2 hours at 30 degree C. MALDI-TOF was used for detection.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant PHD1 / EGLN2 protein gel 10% SDS-PAGE Coomassie staining MW: 44.9 kDa Purity: >90%)
SDS-Page
(Recombinant PHD1 / EGLN2 protein gel 10% SDS-PAGE Coomassie staining MW: 44.9 kDa Purity: >90%)
Testing Data
(MALDI-TOF for Recombinant PHD1 / EGLN2 protein 3 uM HIF-1a peptide (biotin-DLDLEALAPYIPADDDFQL) was incubated with 300 nM PHD1 / EGLN2 protein in a 30 ul reaction containing 20 mM Tris-HCl pH 7.5, 5 mM KCl, 1.5 mM MgCl2, 1 mM DTT, 100 uM 2-oxoglutarate, 100 uM ascorbate and 50 uM (NH4)2Fe(SO4)2-6H20 for 2 hr. at 30 degree C. Single 3 uM HIF-1a peptide was used as a negative control. MALDI-TOF was used for detection.)
Testing Data
(MALDI-TOF for Recombinant PHD1 / EGLN2 protein 3 uM HIF-1a peptide (biotin-DLDLEALAPYIPADDDFQL) was incubated with 300 nM PHD1 / EGLN2 protein in a 30 ul reaction containing 20 mM Tris-HCl pH 7.5, 5 mM KCl, 1.5 mM MgCl2, 1 mM DTT, 100 uM 2-oxoglutarate, 100 uM ascorbate and 50 uM (NH4)2Fe(SO4)2-6H20 for 2 hr. at 30 degree C. Single 3 uM HIF-1a peptide was used as a negative control. MALDI-TOF was used for detection.)
Related Product Information for PHD1 recombinant protein
Short Description: Recombinant human PHD1/EGLN2 protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_542770.2) with an N-terminal DYKDDDDK tag. The molecular weight of the protein is 44.9 kDa. It is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Background: Prolyl Hydroxylase Domain-Containing Protein 1 (PHD1) or Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2), also known as HIF-PH1 or EIT6, is a prolyl hydroxylase. PHD3 is a cellular oxygen sensor that catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins under normoxic conditions. It can hydroxylate a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A, also HIF2A. PHD1 has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. PHD1 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle. It also regulates susceptibility to normoxic oxidative neuronal death. PHD1 links oxygen sensing to cell cycle and primary cilia formation by hydroxylating the critical centrosome component CEP192 which promotes its ubiquitination and subsequent proteasomal degradation. Besides, PHD1 can hydroxylate IKBKB, mediating NF-kappaB activation in hypoxic conditions. Its target proteins are preferentially recognized via a LXXLAP motif.
Background: Prolyl Hydroxylase Domain-Containing Protein 1 (PHD1) or Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2), also known as HIF-PH1 or EIT6, is a prolyl hydroxylase. PHD3 is a cellular oxygen sensor that catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins under normoxic conditions. It can hydroxylate a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A, also HIF2A. PHD1 has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. PHD1 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle. It also regulates susceptibility to normoxic oxidative neuronal death. PHD1 links oxygen sensing to cell cycle and primary cilia formation by hydroxylating the critical centrosome component CEP192 which promotes its ubiquitination and subsequent proteasomal degradation. Besides, PHD1 can hydroxylate IKBKB, mediating NF-kappaB activation in hypoxic conditions. Its target proteins are preferentially recognized via a LXXLAP motif.
Product Categories/Family for PHD1 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,168 Da
NCBI Official Full Name
egl nine homolog 2
NCBI Official Synonym Full Names
egl-9 family hypoxia-inducible factor 2
NCBI Official Symbol
EGLN2
NCBI Official Synonym Symbols
EIT6; PHD1; HPH-1; HPH-3; HIFPH1; HIF-PH1
NCBI Protein Information
egl nine homolog 2; estrogen-induced tag 6; HIF-prolyl hydroxylase 1; hypoxia-inducible factor prolyl hydroxylase 1; prolyl hydroxylase domain-containing protein 1
Protein Family
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Product Notes
The PHD1 (Catalog #AAA389123) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PHD1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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