Rabbit anti-Rat PHEX Polyclonal Antibody | anti-PHEX antibody

PHEX (PEX, Phosphate-regulating Gene with Homologies to Endopeptidases on the X Chromosome)

Cat. Num. AAA611265

• Gene Names: PHEX; HYP; PEX; XLH; HPDR; HYP1; LXHR; HPDR1
• Reactivity: Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: PHEX; Polyclonal Antibody; PHEX (PEX; Phosphate-regulating Gene with Homologies to Endopeptidases on the X Chromosome); Anti -PHEX (PEX; anti-PHEX antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes PHEX. No significant sequence homology of PHEX11-P exists with NEP, NEPLs or other M13-family proteases. Species Sequence Homology
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.1% BSA, 40% glycerol.

• Applicable Applications for anti-PHEX antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1-10ug/ml using ECL.; ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
• Immunogen: Synthetic peptide sequence consisting of 17aa within the extracellular, C-temrinus of rat PHEX (1) (KLH coupled).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-PHEX antibody

The amyloid beta-peptide (Ab) of 39-43aa (Ab40, Ab42, Ab43) is constitutively produced in brain upon proteolysis of the b-amyloid precursor protein (APP). In the young and healthy humans, Ab is rapidly catabolized before it can be deposited in the brain. However, upon aging or the onset of familial Alzheimer's Disease, alterations in either synthesis or degradation/clearance of Ab may contribute to amyloid depositions in the brain. Ab is susceptible to a number of in vivo and in vitro proteases like cathepsin-D and M-13 metalloproteases. The M-13 family includes several members of zinc-dependent proteases like damage-induced neuronal endopeptidase (DINE), product of phosphate regulating gene with homologies to endopeptidases on the X chromosome (PHEX), neutral endopeptidase 24.11 or neprilysin (NEP) and neprilysin-like proteases (NEPLs). NEPLs (alpha, beta, gamma) arise from the alternative splicing of a single NELPS gene and show ~54% sequence homology. M13 members are generally type II transmembrane proteins consisting of a single polypeptide chain with Zinc binding HEXXH motif, a short cytoplasmic tail, a transmembrane segment and an extra-cytoplasmic domain containing the enzyme active site. Phosphate regulating gene with homologies to endopeptidases on the X chromosome (PHEX) (formerly PEX) is a zinc-containing, type II integral membrane glycoprotein (~110kD; mouse/rat/human: 749aa; chromosome xp22.2-p22.1) with structural resemblance to M13 NEP proteases. In contrast to NEP, PHEX has very narrow substrate specificity and hydrolyzes parathroid hormone related peptide (PTHrP107-139). Compared to NEP, PHEX can proteolyze Ab40 (but not Ab42), at a very low rate and to a very poor extent and therefore is not a major in vivo peptidase for Ab. However, it might degrade Ab in Alzheimer patients where Ab is accumulated in excess and an Ab-degradative pathway, alternative to NEP, exists. PHEX is expressed lymphocytes and fetal brain but not in adult brain, placenta, skeletal muscle, pancreas, heart, liver and lung. Defects in PHEX are a cause of X-linked hypophosphatemic rickets (HYP). PHEX is implicated in bone and dentin mineralization and renal phosphate reabsorption.

Product Categories/Family for anti-PHEX antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 90403592
• NCBI GeneID: 5251
• NCBI Accession #: NP_000435.3
• NCBI GenBank Nucleotide #: NM_000444.5
• UniProt Accession #: P78562; O00678; Q13646; Q2M325; Q93032; Q99827
• Molecular Weight: 86,474 Da
• NCBI Official Full Name: phosphate-regulating neutral endopeptidase isoform 1
• NCBI Official Synonym Full Names: phosphate regulating endopeptidase homolog, X-linked
• NCBI Official Symbol: PHEX
• NCBI Official Synonym Symbols: HYP; PEX; XLH; HPDR; HYP1; LXHR; HPDR1
• NCBI Protein Information: phosphate-regulating neutral endopeptidase; metalloendopeptidase homolog PEX; X-linked hypophosphatemia protein; vitamin D-resistant hypophosphatemic rickets protein; phosphate regulating gene with homologies to endopeptidases on the X chromosome (hypophosphatemia, vitamin D resistant rickets)
• UniProt Protein Name: Phosphate-regulating neutral endopeptidase
• Protein Family: Phosphate-regulating neutral endopeptidase
• UniProt Gene Name: PHEX
• UniProt Synonym Gene Names: PEX; HYP
• UniProt Entry Name: PHEX_HUMAN

NCBI Description

The protein encoded by this gene is a transmembrane endopeptidase that belongs to the type II integral membrane zinc-dependent endopeptidase family. The protein is thought to be involved in bone and dentin mineralization and renal phosphate reabsorption. Mutations in this gene cause X-linked hypophosphatemic rickets. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

PHEX: Probably involved in bone and dentin mineralization and renal phosphate reabsorption. Defects in PHEX are a cause of hypophosphatemic rickets, X-linked dominant (XLHR). XLHR is an X-linked dominant disorder characterized by impaired phosphate uptake in the kidney, which is likely to be caused by abnormal regulation of sodium phosphate cotransport in the proximal tubules. Clinical manifestations include skeletal deformities, growth failure, craniosynostosis, paravertebral calcifications, pseudofractures in lower extremities, and muscular hypotonia with onset in early childhood. X-linked hypophosphatemic rickets is the most common form of hypophosphatemia with an incidence of 1 in 20000. Belongs to the peptidase M13 family.

Protein type: Membrane protein, integral; Protease; EC 3.4.24.-

Chromosomal Location of Human Ortholog: Xp22.2-p22.1

Cellular Component: Golgi apparatus; integral to plasma membrane; perinuclear region of cytoplasm; endoplasmic reticulum; plasma membrane

Molecular Function: zinc ion binding; metalloendopeptidase activity; aminopeptidase activity

Biological Process: cell-cell signaling; protein modification process; organophosphate metabolic process; proteolysis; skeletal development; bone mineralization

Disease: Hypophosphatemic Rickets, X-linked Dominant