Rabbit anti-Rat Myosin Light Chain Kinase 2 Polyclonal Antibody | anti-MYLK2 antibody

Myosin Light Chain Kinase 2 (MYLK2) Polyclonal Antibody

Cat. Num. AAA2032126

• Gene Names: Mylk2; Klmc; Mlck
• Reactivity: Rat
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
• Purity: Affinity Chromatography
• Synonyms: Myosin Light Chain Kinase 2; Polyclonal Antibody; Myosin Light Chain Kinase 2 (MYLK2) Polyclonal Antibody; KMLC; skMLCK; MLCK2; Skeletal Muscle Myosin Light Chain Kinase; Myosin light chain kinase 2; skeletal/cardiac muscle; anti-MYLK2 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against MYLK2. It has been selected for its ability to recognize MYLK2 in immunohistochemical staining and western blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence Length: 1845

• Applicable Applications for anti-MYLK2 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
• Application Notes: Western Blot: 1:50-400; Immunohistochemistry in formalin fixed frozen section: 1:50-500; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunoglobulin Type: IgG
• Conjugate: No Conjugate
• Cross Reactivity: Rat
• Organism Species: Rat
• Fragment: MYLK2 (Ala327~Thr536)
• Quality Control: Content: The quality control contains recombinant MYLK2 (Ala327~Thr536) disposed in loading buffer.; Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.; 5uL per well when used in enhanced chemilumescent (ECL).; Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.; Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2071443
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 17105364
• NCBI GeneID: 117558
• NCBI Accession #: NP_476557.1
• NCBI GenBank Nucleotide #: NM_057209.1
• UniProt Accession #: P20689
• Molecular Weight: 65,816 Da
• NCBI Official Full Name: myosin light chain kinase 2, skeletal/cardiac muscle
• NCBI Official Synonym Full Names: myosin light chain kinase 2
• NCBI Official Symbol: Mylk2
• NCBI Official Synonym Symbols: Klmc; Mlck
• NCBI Protein Information: myosin light chain kinase 2, skeletal/cardiac muscle
• UniProt Protein Name: Myosin light chain kinase 2, skeletal/cardiac muscle
• Protein Family: Myosin light chain kinase
• UniProt Gene Name: Mylk2
• UniProt Synonym Gene Names: MLCK2

NCBI Description

kinase; phosphorylates a serine in the N-terminus of a myosin light chain [RGD, Feb 2006]

Uniprot Description

skMLCK: a calcium/calmodulin dependent kinase of the MLCK family. A myosin light chain kinase that is exclusively expressed in adult skeletal muscle.

Protein type: CAMK group; EC 2.7.11.18; Kinase, protein; MLCK family; Motility/polarity/chemotaxis; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 3q41

Cellular Component: cell soma; cytoplasm; dendrite; dendritic spine; nucleus; postsynaptic density; synaptic vesicle; terminal bouton

Molecular Function: ATP binding; calmodulin binding; myosin light chain binding; myosin light chain kinase activity

Biological Process: cardiac muscle morphogensis; muscle contraction; neuromuscular synaptic transmission; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of fast-twitch skeletal muscle contraction; protein amino acid phosphorylation; protein autophosphorylation; regulation of cell cycle; regulation of MAPK cascade; regulation of muscle contraction; regulation of muscle filament sliding; regulation of neuronal synaptic plasticity; skeletal muscle satellite cell differentiation; striated muscle contraction; synaptic vesicle transport; water transport