Rabbit PHAP1 / ANP32A Polyclonal Antibody | anti-PHAP1 antibody

Rabbit Polyclonal to Human PHAP1 / ANP32A

Cat. Num. AAA240833

• Gene Names: ANP32A; LANP; MAPM; PP32; HPPCn; PHAP1; PHAPI; I1PP2A; C15orf1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: PHAP1 / ANP32A; Polyclonal Antibody; Rabbit Polyclonal to Human PHAP1 / ANP32A; Anti-PHAP1 / ANP32A Antibody (C-Terminus) IHC-plus; ANP32A; Hepatopoietin Cn; I1PP2A; LANP; HPPCn; PHAP1; PHAPI; MAPM; PP32; C15orf1; Mapmodulin; Human PHAP1; anti-PHAP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Synthetic peptide corresponding to amino acids at carboxy terminus of human PHAP I
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 249

• Applicable Applications for anti-PHAP1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (10 ug/ml), WB (0.5 - 2 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic peptide corresponding to amino acids at carboxy terminus of human PHAP I.
• Immunogen: PHAP1 / ANP32A antibody was raised against synthetic peptide corresponding to amino acids at carboxy terminus of human PHAP I.
• Antigen Modification: C-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-ANP32A antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of PHAP I in Mouse Small Intestine cells with PHAP I antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot of PHAP I expression in human Raji cell (A), mouse (B) and rat (C) testis tissue lysates with PHAP I antibody at 1 ug/ml.)

Related Product Information for anti-PHAP1 antibody

Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis.

NCBI and Uniprot Product Information

• NCBI GI #: 5453880
• NCBI GeneID: 8125
• NCBI Accession #: NP_006296.1
• NCBI GenBank Nucleotide #: NM_006305.3
• UniProt Accession #: P39687; Q53FK4; Q5J8L8; Q7M4N6; B2R6T4
• Molecular Weight: 28,585 Da
• NCBI Official Full Name: acidic leucine-rich nuclear phosphoprotein 32 family member A
• NCBI Official Synonym Full Names: acidic (leucine-rich) nuclear phosphoprotein 32 family, member A
• NCBI Official Symbol: ANP32A
• NCBI Official Synonym Symbols: LANP; MAPM; PP32; HPPCn; PHAP1; PHAPI; I1PP2A; C15orf1
• NCBI Protein Information: acidic leucine-rich nuclear phosphoprotein 32 family member A; mapmodulin; hepatopoietin Cn; acidic nuclear phosphoprotein pp32; leucine-rich acidic nuclear protein; putative HLA-DR-associated protein I; inhibitor-1 of protein phosphatase-2A; cerebellar leucine rich acidic nuclear protein; putative human HLA class II associated protein I; potent heat-stable protein phosphatase 2A inhibitor I1PP2A
• UniProt Protein Name: Acidic leucine-rich nuclear phosphoprotein 32 family member A
• UniProt Gene Name: ANP32A
• UniProt Synonym Gene Names: C15orf1; LANP; MAPM; PHAP1; pp32; LANP; PHAPI
• UniProt Entry Name: AN32A_HUMAN

Uniprot Description

ANP32A: Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase- independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1- mediated transcriptional repression. Belongs to the ANP32 family.

Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 15q23

Cellular Component: nucleoplasm; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; gene expression; nucleocytoplasmic transport