Mouse anti-Human PHAP1 / ANP32A Monoclonal Antibody | anti-PHAP1 antibody

Mouse Monoclonal [clone 2G11-4A5] (IgG1,k) to Human PHAP1 / ANP32A

Cat. Num. AAA249026

• Gene Names: ANP32A; LANP; MAPM; PP32; HPPCn; PHAP1; PHAPI; I1PP2A; C15orf1
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: PHAP1 / ANP32A; Monoclonal Antibody; Mouse Monoclonal [clone 2G11-4A5] (IgG1; k) to Human PHAP1 / ANP32A; Anti-PHAP1 / ANP32A Antibody (clone 2G11-4A5) IHC-plus; ANP32A; Hepatopoietin Cn; I1PP2A; LANP; HPPCn; PHAP1; PHAPI; MAPM; PP32; C15orf1; Mapmodulin; Human PHAP1; anti-PHAP1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 2G11-4A5
• Specificity: Human PHAP1
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2
• Concentration: 0.3 mg/ml (varies by lot)
• Sequence Length: 249

• Applicable Applications for anti-PHAP1 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB (1:500 - 1:1000); Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Western Blot using cell line lysates and the recombinant protein used as the immunogen.
• Target Species: Human
• Immunogen Description: ANP32A (AAH07200, 1 a.a. ~ 250 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: PHAP1 / ANP32A antibody was raised against aNP32A (AAH07200, 1 a.a. ~ 250 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C or lower. Aliquot to avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-ANP32A antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-ANP32A antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to ANP32A on HeLa cell. [antibody concentration 10 ug/ml])

Western Blot (WB)

(Western blot of ANP32A expression in HeLa.)

Western Blot (WB)

(Western blot of ANP32A expression in Daudi.)

Related Product Information for anti-PHAP1 antibody

Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis.

NCBI and Uniprot Product Information

• NCBI GI #: 5453880
• NCBI GeneID: 8125
• NCBI Accession #: NP_006296.1
• NCBI GenBank Nucleotide #: NM_006305.3
• UniProt Accession #: P39687; Q53FK4; Q5J8L8; Q7M4N6; B2R6T4
• Molecular Weight: 28,585 Da
• NCBI Official Full Name: acidic leucine-rich nuclear phosphoprotein 32 family member A
• NCBI Official Synonym Full Names: acidic (leucine-rich) nuclear phosphoprotein 32 family, member A
• NCBI Official Symbol: ANP32A
• NCBI Official Synonym Symbols: LANP; MAPM; PP32; HPPCn; PHAP1; PHAPI; I1PP2A; C15orf1
• NCBI Protein Information: acidic leucine-rich nuclear phosphoprotein 32 family member A; mapmodulin; hepatopoietin Cn; acidic nuclear phosphoprotein pp32; leucine-rich acidic nuclear protein; putative HLA-DR-associated protein I; inhibitor-1 of protein phosphatase-2A; cerebellar leucine rich acidic nuclear protein; putative human HLA class II associated protein I; potent heat-stable protein phosphatase 2A inhibitor I1PP2A
• UniProt Protein Name: Acidic leucine-rich nuclear phosphoprotein 32 family member A
• UniProt Gene Name: ANP32A
• UniProt Synonym Gene Names: C15orf1; LANP; MAPM; PHAP1; pp32; LANP; PHAPI
• UniProt Entry Name: AN32A_HUMAN

Uniprot Description

ANP32A: Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase- independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1- mediated transcriptional repression. Belongs to the ANP32 family.

Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 15q23

Cellular Component: nucleoplasm; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; gene expression; nucleocytoplasmic transport