Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PHACTR1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3s.)
Rabbit PHACTR1 Polyclonal Antibody | anti-PHACTR1 antibody
PHACTR1 Rabbit pAb
Gene Names
PHACTR1; RPEL; RPEL1; dJ257A7.2
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity purification
Synonyms
PHACTR1; Polyclonal Antibody; PHACTR1 Rabbit pAb; RPEL; RPEL1; EIEE70; dJ257A7.2; anti-PHACTR1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Applicable Applications for anti-PHACTR1 antibody
Western Blot (WB)
Application Notes
WB: 1:1000-1:2000
Immunogen
Recombinant protein of human PHACTR1.
Positive Samples
C6, SH-SY5Y, U-251MG, U-87MG, Mouse brain, Rat brain
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PHACTR1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PHACTR1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3s.)
Related Product Information for anti-PHACTR1 antibody
Background: The protein encoded by this gene is a member of the phosphatase and actin regulator family of proteins. This family member can bind actin and regulate the reorganization of the actin cytoskeleton. It plays a role in tubule formation and in endothelial cell survival. Polymorphisms in this gene are associated with susceptibility to myocardial infarction, coronary artery disease and cervical artery dissection. Alternative splicing of this gene results in multiple transcript variants.
Product Categories/Family for anti-PHACTR1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66,308 Da
NCBI Official Full Name
phosphatase and actin regulator 1
NCBI Official Synonym Full Names
phosphatase and actin regulator 1
NCBI Official Symbol
PHACTR1
NCBI Official Synonym Symbols
RPEL; RPEL1; dJ257A7.2
NCBI Protein Information
phosphatase and actin regulator 1; RPEL repeat containing 1
UniProt Protein Name
Phosphatase and actin regulator 1
Protein Family
UniProt Gene Name
PHACTR1
UniProt Synonym Gene Names
KIAA1733; RPEL1
UniProt Entry Name
PHAR1_HUMAN
Uniprot Description
PHACTR1: Belongs to the phosphatase and actin regulator family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 6p24.1
Cellular Component: synapse; cell junction; cytosol; nucleus
Molecular Function: protein phosphatase type 1 regulator activity; protein phosphatase inhibitor activity; actin binding; protein phosphatase 1 binding
Biological Process: actomyosin structure organization and biogenesis; negative regulation of catalytic activity; actin cytoskeleton reorganization; stress fiber formation; cell motility involved in cell locomotion
Research Articles on PHACTR1
Similar Products
Product Notes
The PHACTR1 phactr1 (Catalog #AAA9143569) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PHACTR1 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PHACTR1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000-1:2000. Researchers should empirically determine the suitability of the PHACTR1 phactr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHACTR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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