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Western Blot (WB) (Western blot analysis of PHACS expression in Jurkat (A), mouse testis (B), rat kidney (C) whole cell lysates.)

Rabbit anti-Human PHACS Polyclonal Antibody | anti-ACCS antibody

Anti-PHACS Antibody

Gene Names
ACCS; ACS; PHACS
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
PHACS; Polyclonal Antibody; Anti-PHACS Antibody; 1-aminocyclopropane-1-carboxylate synthase-like protein 1; ACC synthase-like protein 1; anti-ACCS antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of PHACS protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
501
Applicable Applications for anti-ACCS antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
WB: 1/500 - 1/2000; IHC: 1/50 - 1/200; IF/ICC: 1/50 - 1/200
Immunogen
Recombinant full length protein of human PHACS
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of PHACS expression in Jurkat (A), mouse testis (B), rat kidney (C) whole cell lysates.)

Western Blot (WB) (Western blot analysis of PHACS expression in Jurkat (A), mouse testis (B), rat kidney (C) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of PHACS staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of PHACS staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunofluorescence (IF)

(Immunofluorescent analysis of PHACS staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)

Immunofluorescence (IF) (Immunofluorescent analysis of PHACS staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Related Product Information for anti-ACCS antibody
Rabbit polyclonal antibody to PHACS

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17,598 Da
NCBI Official Full Name
1-aminocyclopropane-1-carboxylate synthase-like protein 1
NCBI Official Synonym Full Names
1-aminocyclopropane-1-carboxylate synthase homolog (inactive)
NCBI Official Symbol
ACCS
NCBI Official Synonym Symbols
ACS; PHACS
NCBI Protein Information
1-aminocyclopropane-1-carboxylate synthase-like protein 1
UniProt Protein Name
1-aminocyclopropane-1-carboxylate synthase-like protein 1
UniProt Gene Name
ACCS
UniProt Synonym Gene Names
PHACS; ACC synthase-like protein 1
UniProt Entry Name
1A1L1_HUMAN

Uniprot Description

ACCS: Does not catalyze the synthesis of 1-aminocyclopropane- 1-carboxylate but is capable of catalyzing the deamination of L- vinylglycine. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Protein type: Lyase

Chromosomal Location of Human Ortholog: 11p11

Molecular Function: 1-aminocyclopropane-1-carboxylate synthase activity; protein binding; protein homodimerization activity; pyridoxal phosphate binding

Biological Process: biosynthetic process

Research Articles on ACCS

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Product Notes

The ACCS accs (Catalog #AAA8245295) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PHACS Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PHACS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1/500 - 1/2000; IHC: 1/50 - 1/200; IF/ICC: 1/50 - 1/200. Researchers should empirically determine the suitability of the ACCS accs for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHACS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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