Rabbit anti-Human PH4 Polyclonal Antibody

Anti-PH4 (Transmembrane Prolyl 4-hydroxylase, P4H-TM, Hypoxia-inducible Factor Prolyl Hydroxylase 4, HIF-PH4, HIF-prolyl Hydroxylase 4, HPH-4, P4HTM, PH-4)

Cat. Num. AAA630320

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified
• Synonyms: PH4; Polyclonal Antibody; Anti-PH4 (Transmembrane Prolyl 4-hydroxylase; P4H-TM; Hypoxia-inducible Factor Prolyl Hydroxylase 4; HIF-PH4; HIF-prolyl Hydroxylase 4; HPH-4; P4HTM; PH-4); Transmembrane Prolyl 4-hydroxylase; PH-4; anti-PH4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PH-4.
• Purity/Purification: Affinity Purified
• Form/Format: Supplied as a liquid in PBS, 0.1% BSA, 0.05% sodium azide.
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-PH4 antibody: ELISA; Western Blot
• Application Notes: Recommended Dilutions: Western Blot: 1-5ug/ml (chemiluminescence); ELISA: 1:10,000-1:100,000; 50-100ng control peptide/well
• Immunogen: Synthetic peptide corresponding to 17aa of human PH-4, C-terminal.
• Source Information: Human
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.; Shipping Temperature: Blue Ice

Related Product Information for anti-PH4 antibody

Oxygen is absolutely critical for the survival of mammalian cells. Hypoxia induced factor (HIF) is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. There are 3-types of alpha subunits (HIF1-3alpha) and one HIF-beta subunits. However, the three HIF-alpha subunits are regulated by oxygen in a similar fashion, i.e. by regulated stabilization of the alpha-subunits. Under normal conditions, HIF-alpha Prolines are hydroxylated at Pro-402 and Pro-564. This allows binding of von Hippel-Lindau (VHL), the substrate recognition component of the E3 ubiquinated ligase complex, subsequent ubiquitination and degradation of HIF-alpha by the proteasome. Under hypoxic conditions, hydroxylation of HIF-alpha is inhibited and this prevents HIF-alpha degradation. The enzymes responsible for HIF-hydroxylation are known as HIF-prolyl hydroxylases (PHD1- 3 or HPH1, HPH2, and HPH3). The three PHDs have been identified to hydroxylate the motif, LXXLAP* with *P being the hydroxyproline.

A novel putative proline hydroxylase, PH-4, with an N-terminal EF-hand motif and a C-terminal catalytic domain with 1 transmembrane domain has been identified in endoplasmic reticulum. PH-4 showed approx. 10-15% homology with the collagen prolyl 4-hydroxylases, PH-alpha I, and PH-alpha II. Human PH-4 (502aa, chromosome 3pp21.3) is highly expressed in most tissues. Like PHD1-3, PH-4 suppressed the HIF transactivation activity, dependent on the consensus oxygen-dependent degradation domain (ODDD) praline residues. Ph-4 levels correlated with the cellular HIF concentration. Therefore, PH-4 may also be involved in cellular oxygen sensing mechanism.

Product Categories/Family for anti-PH4 antibody

Abs to Proteins