Rabbit anti-Human, Mouse PGP9.5 Polyclonal Antibody | anti-PGP9.5 antibody

PGP9.5, NT (Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1, UCH-L1, Ubiquitin Thiolesterase L1, Neuron Cytoplasmic Protein 9.5, UCHL1) (MaxLight 750)

Cat. Num. AAA6495024

• Gene Names: UCHL1; NDGOA; PARK5; PGP95; SPG79; PGP9.5; Uch-L1; HEL-117; PGP 9.5; HEL-S-53
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: PGP9.5; Polyclonal Antibody; NT (Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1; UCH-L1; Ubiquitin Thiolesterase L1; Neuron Cytoplasmic Protein 9.5; UCHL1) (MaxLight 750); anti-PGP9.5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PGP9.5. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-PGP9.5 antibody: FLISA, Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 16-46 in the N-terminal region of human PGP9.5, UniProt Accession #P09936. Species sequence homology: bovine and porcine
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of UCHL1 (arrow) of 293 cell lysates (2ug/lane) usingMBS6010792. Lane 1: non-transfected Lane 2: transiently transfected with the UCHL1 gene (Origene Technologies).)

Western Blot (WB)

(Western Blot analysis of UCHL1-V31 in CEM(lane 1), Jurkat (lane 2), Y79(lane 3) cell line and mouse brain tissue(lane 4) lysates (35ug/lane) usingMBS6010792. UCHL1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue usingMBS6010792, followed by peroxidase-conjugated secondary antibody, followed by DAB staining. This data demonstrates the use of MBS6010792 for immunohistochemistry)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of NCI-H460 cell using MBS6010792 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of NCI-H460 cells using MBS6010792 (right histogram) compared to a negative control cell (left histogram). IgG (FITC) goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PGP9.5 antibody

UCHL1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons (Doran et al., 1983 [PubMed 6343558]).

Product Categories/Family for anti-PGP9.5 antibody

Antibodies; Ubiquitin

References

1. Maraganore, D.M., et al., Mov Disord 18(6):631-636 (2003). 2. Nishikawa, K., et al., Biochem. Biophys. Res. Commun. 304(1):176-183 (2003). 3. Liu, Y., et al., Cell 111(2):209-218 (2002). 4. Caballero, O.L., et al., Oncogene 21(19):3003-3010 (2002). 5. Saigoh, K., et al., Nat. Genet. 23(1):47-51 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 21361091
• NCBI GeneID: 7345
• NCBI Accession #: NP_004172
• NCBI GenBank Nucleotide #: NM_004181.4
• UniProt Accession #: P09936; Q4W5K6; Q71UM0
• Molecular Weight: 24.8 kDa (223 aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase isozyme L1
• NCBI Official Synonym Full Names: ubiquitin C-terminal hydrolase L1
• NCBI Official Symbol: UCHL1
• NCBI Official Synonym Symbols: NDGOA; PARK5; PGP95; SPG79; PGP9.5; Uch-L1; HEL-117; PGP 9.5; HEL-S-53
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase isozyme L1
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase isozyme L1
• UniProt Gene Name: UCHL1
• UniProt Synonym Gene Names: UCH-L1; PGP9.5
• UniProt Entry Name: UCHL1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]

Uniprot Description

UCHL1: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. Monomer. Homodimer. Interacts with SNCA. Interacts with COPS5. Found in neuronal cell bodies and processes throughout the neocortex. Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. Belongs to the peptidase C12 family.

Protein type: Cell development/differentiation; EC 3.4.19.12; Ligase; Protease; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; cell soma; axon; cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; omega peptidase activity; ubiquitin binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; alpha-2A adrenergic receptor binding; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; cell proliferation; negative regulation of MAP kinase activity; protein deubiquitination; axon transport of mitochondrion; axon target recognition; eating behavior; neuromuscular process; sensory perception of pain; muscle fiber development; adult walking behavior

Disease: Neurodegeneration With Optic Atrophy, Childhood-onset; Parkinson Disease 5, Autosomal Dominant