Testing Data
(Figure: Detection of natural human Clusterin and DYKDDDDK-tagged overexpressed Clusterin in transfected HEK 293T cells. Method: Endogenous human Clusterin and recombinant DYKDDDDK-tagged Clusterin from cell lysates were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with a 1:2'000 dilution of the CL1-9 antibody on the left or with an anti-DYKDDDDK epitope-tag antibody on the right. Proteins were visualized using an HRP-conjugated anti-mouse IgG antibody and a chemiluminescence detection system.)
Mouse anti-Human Clusterin Monoclonal Antibody | anti-CLU antibody
Clusterin (human) monoclonal antibody (CLI-9)
Suggested dilutions/conditions may not be available for all applications.
Stable for at least 6 months after receipt when stored at +4 degree C undiluted. Avoid freeze/thaw cycles.
Testing Data
(Figure: Detection of natural human Clusterin and DYKDDDDK-tagged overexpressed Clusterin in transfected HEK 293T cells. Method: Endogenous human Clusterin and recombinant DYKDDDDK-tagged Clusterin from cell lysates were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with a 1:2'000 dilution of the CL1-9 antibody on the left or with an anti-DYKDDDDK epitope-tag antibody on the right. Proteins were visualized using an HRP-conjugated anti-mouse IgG antibody and a chemiluminescence detection system.)
Testing Data
(Figure: Detection of natural human Clusterin and DYKDDDDK-tagged overexpressed Clusterin in transfected HEK 293T cells. Method: Endogenous human Clusterin and recombinant DYKDDDDK-tagged Clusterin from cell lysates were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with a 1:2'000 dilution of the CL1-9 antibody on the left or with an anti-DYKDDDDK epitope-tag antibody on the right. Proteins were visualized using an HRP-conjugated anti-mouse IgG antibody and a chemiluminescence detection system.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.[provided by RefSeq, May 2011]
Uniprot Description
CLU: Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress- induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. Belongs to the clusterin family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Mitochondrial; Secreted; Apoptosis
Chromosomal Location of Human Ortholog: 8p21-p12
Cellular Component: cell surface; cytoplasm; cytosol; endoplasmic reticulum; extracellular matrix; extracellular region; extracellular space; Golgi apparatus; growth cone; intracellular; mitochondrial membrane; mitochondrion; nucleus; perinuclear region of cytoplasm; protein complex
Molecular Function: ATPase activity; chaperone binding; misfolded protein binding; protein binding; protein N-terminus binding; ubiquitin protein ligase binding
Biological Process: activation of NF-kappaB transcription factor; aging; blood coagulation; cell morphogenesis; chaperone-mediated protein complex assembly; complement activation; complement activation, classical pathway; endocrine pancreas development; innate immune response; lipid metabolic process; microglial cell activation; myelin maintenance in the central nervous system; negative regulation of protein homooligomerization; neurite morphogenesis; platelet activation; platelet degranulation; positive regulation of apoptosis; positive regulation of cell differentiation; positive regulation of cell proliferation; positive regulation of nitric oxide biosynthetic process; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of tumor necrosis factor production; protein import; protein stabilization; release of cytochrome c from mitochondria; response to light stimulus; response to misfolded protein; response to virus; reverse cholesterol transport
Research Articles on CLU
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Product Notes
The CLU clu (Catalog #AAA565137) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Clusterin (human) monoclonal antibody (CLI-9) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Clusterin can be used in a range of immunoassay formats including, but not limited to, Immunocyotochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP), Western Blot (WB). Western Blot (1:2,000) Suggested dilutions/conditions may not be available for all applications. Researchers should empirically determine the suitability of the CLU clu for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Clusterin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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